基于ISSR分析的黄连种质遗传多样性研究
[Genetic diversity of Coptis chinensis germplasm based on ISSR analysis].
作者信息
Chen Da-xia, Li Long-yun, Peng Rui, Qu Xian-you
机构信息
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Dec;31(23):1937-40.
OBJECTIVE
To detemine the genetic diversity of Coptis chinensis.
METHOD
32 germplasmic resources of C. chinensis were analyzed by ISSR molecular markers. To make up the systematic diagram of genetic relationship by TREECONW software and clustered by UPGMA method.
RESULT
A total of 106 ISSR bands were scored, among which 51 were polymorphic bands. The average percentage of polymorphic bands was 48. 1%. Genetic similarity coefficient were changed from 0. 825 8 to 0. 935 1. By cluster analysis, the geographical distribution is not very obvious, but it was also showed some of the C. chinensis from the same region were in the same group which presented the law of geographical distribution in the tested materials.
CONCLUSION
Different germplasms diversity of C. chinensis is low and the relationship of C. chinensis is close.
目的
测定黄连的遗传多样性。
方法
采用ISSR分子标记对32份黄连种质资源进行分析，利用TREECONW软件构建遗传关系系统图，并采用UPGMA法进行聚类。
结果
共记录到106条ISSR条带，其中多态性条带51条，多态性条带平均百分率为48.1%。遗传相似系数在0.825 8至0.935 1之间。通过聚类分析，地理分布不太明显，但也显示出部分来自同一地区的黄连在同一组中，在供试材料中呈现出地理分布规律。
结论
黄连不同种质间的遗传多样性较低，亲缘关系较近。
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