Ibbotson J P, Lowes J R, Chahal H, Gaston J S, Life P, Kumararatne D S, Sharif H, Alexander-Williams J, Allan R N
Department of Infection, University of Birmingham, UK.
Clin Exp Immunol. 1992 Feb;87(2):224-30. doi: 10.1111/j.1365-2249.1992.tb02979.x.
Culture studies have suggested that Mycobacterium paratuberculosis may play a role in the aetiology of Crohn's disease. However, evidence of sensitization to mycobacterial antigens amongst patients with Crohn's disease has not yet been adequately demonstrated. Previous studies of cell-mediated immunity (CMI) in Crohn's disease were restricted to responses of peripheral blood mononuclear cells (PBMC) to mycobacterial antigens. In this study we have investigated the proliferative responses of both PBMC and mesenteric lymph node mononuclear cells (MLNMC) to a range of mycobacterial and non-mycobacterial antigens. There was no evidence of specific sensitization in the responses of MLNMC and PBMC from patients with inflammatory bowel disease (IBD) to the mycobacterial antigens. However, anergy to M. paratuberculosis could not be excluded. IBD MLNMC responses to most antigens were generally greater than those of PBMC, which were often undetectable. When compared with controls, there was evidence of increased CMI to a range of non-mycobacterial antigens, especially Yersinia enterocolitica, amongst both MLNMC and PBMC from patients with Crohn's disease and ulcerative colitis (UC). These results do not provide support to the proposed role of mycobacteria in the pathogenesis of Crohn's disease, but indicate that further investigation may determine a role for bacterial-specific T cell-mediated responses in the pathogenesis of IBD.
培养研究表明，副结核分枝杆菌可能在克罗恩病的病因学中起作用。然而，克罗恩病患者对分枝杆菌抗原致敏的证据尚未得到充分证实。先前关于克罗恩病细胞介导免疫（CMI）的研究仅限于外周血单核细胞（PBMC）对分枝杆菌抗原的反应。在本研究中，我们调查了PBMC和肠系膜淋巴结单核细胞（MLNMC）对一系列分枝杆菌和非分枝杆菌抗原的增殖反应。炎症性肠病（IBD）患者的MLNMC和PBMC对分枝杆菌抗原的反应中没有特异性致敏的证据。然而，不能排除对副结核分枝杆菌无反应。IBD的MLNMC对大多数抗原的反应通常大于PBMC，而PBMC的反应往往无法检测到。与对照组相比，克罗恩病和溃疡性结肠炎（UC）患者的MLNMC和PBMC对一系列非分枝杆菌抗原，尤其是小肠结肠炎耶尔森菌的CMI有增加的证据。这些结果不支持分枝杆菌在克罗恩病发病机制中所提出的作用，但表明进一步研究可能确定细菌特异性T细胞介导的反应在IBD发病机制中的作用。
