Ford D K, da Roza D, Schulzer M
Ann Rheum Dis. 1985 Oct;44(10):701-10. doi: 10.1136/ard.44.10.701.
The [3H]thymidine uptake procedure for measuring lymphocyte responses was applied to lymphocytes derived concurrently from synovial effusions and from peripheral blood. The stimulating antigens were crude preparations of those micro-organisms that are related to the enteritis and the non-gonococcal urethritis that precipitate reactive arthritis. Salmonella, shigella, and campylobacter antigens stimulated synovial but not peripheral blood lymphocytes in eight cases of enteric reactive arthritis. Ureaplasma or chlamydia antigens, or both, stimulated synovial lymphocytes in all 12 cases of sexually transmitted reactive arthritis, whereas peripheral blood lymphocytes were only stimulated in four of the 12 cases. In 14 cases of rheumatoid arthritis reactions to either enteric or ureaplasma/chlamydia antigens were minimal from either synovial or peripheral blood lymphocytes. It is concluded that synovial rather than peripheral blood lymphocytes indicate the microbiological cause of reactive arthritis and that similar studies of lymphocytes from the site of local disease might be productive in other diseases.
用于测量淋巴细胞反应的[3H]胸苷摄取程序应用于同时取自滑膜积液和外周血的淋巴细胞。刺激抗原是与肠炎和引发反应性关节炎的非淋菌性尿道炎相关的微生物粗制品。在8例肠道反应性关节炎患者中，沙门氏菌、志贺氏菌和弯曲杆菌抗原刺激滑膜淋巴细胞，但不刺激外周血淋巴细胞。脲原体或衣原体抗原，或两者，刺激了所有12例性传播反应性关节炎患者的滑膜淋巴细胞，而外周血淋巴细胞仅在12例中的4例中受到刺激。在14例类风湿性关节炎患者中，滑膜或外周血淋巴细胞对肠道或脲原体/衣原体抗原的反应极小。结论是，滑膜淋巴细胞而非外周血淋巴细胞表明了反应性关节炎的微生物病因，并且对局部疾病部位淋巴细胞进行类似研究可能对其他疾病也有成效。
