Ueno Takayuki, Iguro Yoshihumi, Yotsumoto Goichi, Fukumoto Yoshihiro, Nakamura Kazuo, Miyamoto Tadaomi-Alfonso, Sakata Ryuzo
Department of Thoracic and Cardiovascular Surgery, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Resuscitation. 2007 May;73(2):287-95. doi: 10.1016/j.resuscitation.2006.12.011. Epub 2007 Mar 13.
The Myocardial protective effects of taurine (TA) are well known. We investigated the optimal phase of giving taurine to reduce myocardial ischaemia-reperfusion injury in isolated rat hearts.
Isolated rat hearts were subjected to 20 min of global ischaemia followed by 60 min of reperfusion under three different conditions: global ischaemia alone (control group; n=8); pre-ischaemic administration of taurine (pre-TA group; n=8), perfusion with 10 mmol/L taurine for 10 min just before ischaemia; post-ischaemic administration of taurine (post-TA group; n=8), perfusion with 10 mmol/L taurine for the first 10 min of reperfusion. Ventricular functional and biochemical variables, the area at risk (AAR), and infarct size (IS) after reperfusion were compared between groups.
Recovery of ventricular function in the post-TA group was significantly greater than that in the control and pre-TA groups in terms of left ventricular pressure and rate-pressure product. Lipid peroxide product as a marker of oxidant stress in the post-TA group was significantly less than that in the control and pre-TA groups. AAR relative to left ventricular area in the post-TA group was significantly less than that in the control and pre-TA groups. IS relative to AAR in the post-TA group was significantly less than that in the control group.
Taurine administered before or after ischaemia prevents infarction; being a potent free radical scavenging antioxidant, it reduced myocardial injury and provided significantly better functional recovery when given immediately after reperfusion.
牛磺酸（TA）的心肌保护作用已广为人知。我们研究了给予牛磺酸以减轻离体大鼠心脏缺血-再灌注损伤的最佳阶段。
将离体大鼠心脏在三种不同条件下进行20分钟全心缺血，随后再灌注60分钟：单纯全心缺血（对照组；n = 8）；缺血前给予牛磺酸（缺血前TA组；n = 8），在缺血前用10 mmol/L牛磺酸灌注10分钟；缺血后给予牛磺酸（缺血后TA组；n = 8），在再灌注的最初10分钟用10 mmol/L牛磺酸灌注。比较各组再灌注后的心室功能和生化指标、危险面积（AAR）和梗死面积（IS）。
在左心室压力和心率-压力乘积方面，缺血后TA组的心室功能恢复明显优于对照组和缺血前TA组。作为氧化应激标志物的脂质过氧化物产物在缺血后TA组明显少于对照组和缺血前TA组。缺血后TA组相对于左心室面积的AAR明显小于对照组和缺血前TA组。缺血后TA组相对于AAR的IS明显小于对照组。
缺血前后给予牛磺酸可预防梗死；作为一种有效的自由基清除抗氧化剂，在再灌注后立即给予时，它可减轻心肌损伤并提供明显更好的功能恢复。
