Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of Cell, Developmental and Integrative Biology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Adv Exp Med Biol. 2017;975 Pt 2:741-755. doi: 10.1007/978-94-024-1079-2_58.
Perinatal taurine depletion followed by high sugar intake after weaning adversely affects myocardial and arterial pressure function following a myocardial ischemia and reperfusion (IR) insult in adult female rats. This study tests the hypothesis that taurine supplementation ameliorates this adverse effect. Female Sprague-Dawley rats were fed normal rat chow and drank water containing β-alanine from conception until weaning (taurine depletion, TD). After weaning, female offspring were fed normal rat chow and drank either water containing 5% glucose (TDG) or water alone (TDW). At 6-7 weeks of age, half the rats in each group were supplemented with taurine and 1 week later subjected to cardiac IR. Body weight, heart weight, plasma electrolytes, plasma creatinine, blood urea nitrogen, and hematocrit were not significantly different among the four groups. The mean arterial pressures significantly increased in all groups after IR, but values were not significantly different among the four groups. Heart rates were significantly increased after IR only in TDW group. Compared to TDW, TDG displayed increased plasma cardiac injury markers (creatinine kinase-MB, troponin T, and N-terminal prohormone brain natriuretic peptide), increased sympathetic activity, decreased parasympathetic activity, and decreased baroreflex sensitivity after IR. Taurine supplementation completely restored the baroreflex and autonomic dysfunction of TDG to TDW levels and partially decreased myocardial injury after cardiac IR. The present study indicates that in adult female rats, perinatal taurine depletion followed by high sugar intake after weaning exacerbates cardiac IR injury and arterial pressure dysregulation and these adverse effects can be partially prevented by taurine supplementation.
围产期牛磺酸耗竭，随后断奶后高糖摄入，对成年雌性大鼠心肌缺血再灌注（IR）损伤后的心肌和动脉压功能产生不利影响。本研究检验了牛磺酸补充可改善这种不利影响的假设。雌性 Sprague-Dawley 大鼠从受孕开始至断奶期间，给予正常大鼠饲料和含β-丙氨酸的水（牛磺酸耗竭，TD）。断奶后，雌性后代给予正常大鼠饲料和饮用水，其中含有 5%葡萄糖（TDG）或单独饮用水（TDW）。在 6-7 周龄时，每组一半的大鼠接受牛磺酸补充，并在 1 周后进行心脏 IR。体重、心脏重量、血浆电解质、血浆肌酐、血尿素氮和红细胞压积在四组之间无显著差异。所有组的平均动脉压在 IR 后均显著升高，但四组之间无显著差异。IR 后仅 TDW 组的心率显著升高。与 TDW 相比，TDG 显示出增加的血浆心脏损伤标志物（肌酸激酶-MB、肌钙蛋白 T 和 N 端脑利钠肽原）、增加的交感神经活性、降低的副交感神经活性和降低的压力反射敏感性。牛磺酸补充完全恢复了 TDG 的压力反射和自主神经功能障碍，使其恢复到 TDW 水平，并部分降低了心脏 IR 后的心肌损伤。本研究表明，在成年雌性大鼠中，围产期牛磺酸耗竭，随后断奶后高糖摄入，可加重心脏 IR 损伤和动脉压失调，而牛磺酸补充可部分预防这些不利影响。
