Ohta Hitomi, Tanimoto Tadao, Taniai Madoka, Taniguchi Mutsuko, Ariyasu Toshio, Arai Shigeyuki, Ohta Tsunetaka, Fukuda Shigeharu
Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Okayama, Japan.
In Vivo. 2007 Jan-Feb;21(1):25-32.
Endogenous tumor necrosis factor-alpha (TNF-alpha) has a beneficial effect as an activation mediator of host defense against infection by the fungus Candida albicans (C. albicans). However, it is unclear whether exogenous TNF-alpha has a beneficial or detrimental effect against Candida.
The direct effect of TNF-alpha on CO2-induced morphological transformation of C. albicans blastoconidia was examined in vitro and the effect of TNF-alpha was determined in a mouse model of oral candidiasis.
TNF-alpha suppressed hyphal formation from C. albicans blastoconidia directly and dose-dependently, whereas it did not affect the fungal budding rate at concentrations ranging from 0.01 to 10 microg/ml. In vivo, the oral administration of TNF-alpha significantly reduced the C. albicans CFU in tongue tissues of treated mice. Histopathologically, there was a decrease in the number and size of C. albicans fungi in the tongue tissues.
Since orally administered TNF-alpha suppressed fungal burden in the tongue tissue without significant detrimental effects, TNF-alpha has potential as a therapeutic agent against Candida.
内源性肿瘤坏死因子-α（TNF-α）作为宿主抵御白色念珠菌感染的激活介质具有有益作用。然而，外源性TNF-α对念珠菌是有益还是有害尚不清楚。
在体外检测TNF-α对二氧化碳诱导的白色念珠菌芽生孢子形态转化的直接作用，并在口腔念珠菌病小鼠模型中确定TNF-α的作用。
TNF-α直接且剂量依赖性地抑制白色念珠菌芽生孢子形成菌丝，而在0.01至10微克/毫升的浓度范围内，它不影响真菌的出芽率。在体内，口服TNF-α显著降低了治疗小鼠舌组织中的白色念珠菌菌落形成单位。组织病理学上，舌组织中白色念珠菌的数量和大小有所减少。
由于口服TNF-α可抑制舌组织中的真菌负荷且无明显有害影响，TNF-α有潜力作为抗念珠菌的治疗药物。
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