Prenatal Diagnosis Center, Longhua Maternity and Child Healthcare Hospital, Shenzhen, Guangdong, China.
Department of Laboratory Medicine, Lishui Second People's Hospital, Lishui, Zhejiang, China.
Front Immunol. 2023 Dec 14;14:1317809. doi: 10.3389/fimmu.2023.1317809. eCollection 2023.
Hypertension (HTN) and chronic kidney disease (CKD) pose significant global health challenges and often coexist, amplifying cardiovascular risks. Recent attention has turned to the gut mycobiome as a potential factor in their pathophysiology. Our study sought to examine the gut fungal profile in individuals with HTN, CKD, and the concurrent HTN+CKD condition, investigating its connections with serum cytokines, renal function, and blood pressure.
We investigated three distinct participant groups: a cohort of 50 healthy controls (HC), 50 individuals diagnosed with HTN-only, and 50 participants suffering from both HTN and CKD (HTN+CKD). To facilitate our research, we gathered fecal and blood samples and conducted a comprehensive analysis of serum cytokines. Moreover, fungal DNA extraction was conducted with meticulous care, followed by sequencing of the Internal Transcribed Spacer (ITS) region.
HTN+CKD patients displayed distinctive fungal composition with increased richness and diversity compared to controls. In contrast, HTN-only patients exhibited minimal fungal differences. Specific fungal genera were notably altered in HTN+CKD patients, characterized by increased and levels and reduced abundance. Our correlation analyses revealed significant associations between fungal genera and serum cytokines. Moreover, certain fungal taxa, such as and , exhibited positive correlations with renal function, while others, including , , and , were linked to blood pressure, particularly diastolic pressure.
Gut mycobiome dysbiosis in individuals with comorbid HTN and CKD differs significantly from that observed in HTN-only and healthy controls. The interactions between serum cytokines, renal function, and blood pressure emphasize the potential impact of the fungal microbiome on these conditions. Additional research is required to clarify the underlying mechanisms and identify therapeutic opportunities associated with mycobiome dysbiosis in HTN and CKD.
高血压（HTN）和慢性肾脏病（CKD）是全球性的健康挑战，常同时存在，增加心血管风险。最近，人们开始关注肠道真菌组作为其病理生理学的一个潜在因素。我们的研究旨在检查 HTN、CKD 和同时患有 HTN+CKD 的个体的肠道真菌谱，研究其与血清细胞因子、肾功能和血压的关系。
我们研究了三个不同的参与者群体：一个由 50 名健康对照者（HC）、50 名仅患有 HTN 的参与者和 50 名同时患有 HTN 和 CKD 的参与者组成的队列。为了便于研究，我们收集了粪便和血液样本，并对血清细胞因子进行了全面分析。此外，我们小心翼翼地提取真菌 DNA，然后对内部转录间隔区（ITS）进行测序。
与对照组相比，HTN+CKD 患者的真菌组成具有独特的特征，丰富度和多样性增加。相比之下，仅患有 HTN 的患者的真菌差异很小。在 HTN+CKD 患者中，特定的真菌属明显改变，表现为水平升高和水平降低。我们的相关性分析显示，真菌属与血清细胞因子之间存在显著关联。此外，某些真菌类群，如和，与肾功能呈正相关，而其他真菌类群，如、和，与血压呈正相关，尤其是舒张压。
患有 HTN 和 CKD 的合并症患者的肠道真菌组失调与仅患有 HTN 和健康对照者观察到的情况有显著差异。血清细胞因子、肾功能和血压之间的相互作用强调了真菌微生物组对这些疾病的潜在影响。需要进一步研究以阐明潜在机制，并确定与 HTN 和 CKD 中真菌组失调相关的治疗机会。
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