Pm35的染色体定位，Pm35是从小麦草（节节麦）中导入普通小麦（普通小麦）的一个新的抗白粉病基因。
Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.).
作者信息
Miranda L M, Murphy J P, Marshall D, Cowger C, Leath S
机构信息
Department of Crop Science, North Carolina State University, Box 7629, Raleigh, NC 27695, USA.
出版信息
Theor Appl Genet. 2007 May;114(8):1451-6. doi: 10.1007/s00122-007-0530-4. Epub 2007 Mar 14.
A single gene controlling powdery mildew resistance was identified in the North Carolina germplasm line NC96BGTD3 (NCD3) using genetic analysis of F(2) derived lines from a NCD3 X Saluda cross. Microsatellite markers linked to this Pm gene were identified and their most likely order was Xcfd7, 10.3 cM, Xgdm43, 8.6 cM, Xcfd26, 11.9 cM, Pm gene. These markers and the Pm gene were assigned to chromosome 5DL by means of Chinese Spring Nullitetrasomic (Nulli5D-tetra5A) and ditelosomic (Dt5DL) lines. A detached leaf test showed a distinctive disease reaction to six pathogen isolates among the NCD3 Pm gene, Pm2 (5DS) and Pm34 (5DL). An allelism test showed independence between Pm34 and the NCD3 Pm gene. Together, the tests provided strong evidence for the presence of a novel Pm gene in NCD3, and this gene was designated Pm35.
利用NCD3与Saluda杂交产生的F(2)衍生系进行遗传分析，在北卡罗来纳种质系NC96BGTD3（NCD3）中鉴定出一个控制白粉病抗性的单基因。鉴定出与该Pm基因连锁的微卫星标记，其最可能的顺序为：Xcfd7，10.3厘摩，Xgdm43，8.6厘摩，Xcfd26，11.9厘摩，Pm基因。通过中国春缺四体（Nulli5D-四体5A）和双端体（Dt5DL）系，将这些标记和Pm基因定位到5DL染色体上。离体叶片试验表明，NCD3 Pm基因、Pm2（5DS）和Pm34（5DL）对六种病原菌分离物有独特的病害反应。等位性测验表明Pm34与NCD3 Pm基因相互独立。这些试验共同提供了强有力的证据，证明NCD3中存在一个新的Pm基因，该基因被命名为Pm35。
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