Torres A, Torres K, Paszkowski T, Staśkiewicz G J, Maciejewski R
Human Anatomy Department, Medical University of Lublin, Jaczewskiego 4, 20-094, Lublin, Poland.
Surg Endosc. 2007 Oct;21(10):1841-8. doi: 10.1007/s00464-007-9260-6. Epub 2007 Mar 14.
Cytokines are the main mediators of the inflammation and the response to trauma. The purpose of the present study was the comparative assessment in sera of patients with benign adnexal masses treated by laparoscopy or laparotomy of the following proinflammatory and anti-inflammatory cytokines: interleukin (IL)-1beta, IL-6, IL-8, tumor necrosis factor-alpha (TNF-alpha), and IL-10 in the early postoperative period.
A total of 40 patients with benign adnexal masses were studied; 25 of whom underwent laparoscopy and 15, laparotomy. Blood serum concentration of IL-1beta, IL-6, IL-8, TNF-alpha, and IL-10 were measured by commercially available ELISA assays before and 4 h, 24 h, and 48 h after the operation.
Concentrations of IL-6 were significantly increased in both groups at 4 h, 24 h, and 48 h after the surgery; levels of IL-10 showed a significant increase 4 h and 24 h after the operation; an increase in IL-1beta levels was observed only after laparotomy; no significant variations were observed in serum levels of IL-8; the postoperative increase of IL-1beta, IL-6, and IL-10 levels was more pronounced in patients undergoing laparotomy than in those treated laparoscopically; length of the surgical procedure, amount of CO2 used, tumor diameter, age, and body mass index (BMI) of the patients did not influence the postoperative patterns of the studied cytokines.
Systemic cytokine response after operations for benign adnexal masses depends on the degree of the surgical trauma, and is less pronounced in patients undergoing laparoscopy.
细胞因子是炎症和创伤反应的主要介质。本研究的目的是比较腹腔镜手术或开腹手术治疗的良性附件肿块患者术后早期血清中以下促炎和抗炎细胞因子的水平：白细胞介素（IL）-1β、IL-6、IL-8、肿瘤坏死因子-α（TNF-α）和IL-10。
共研究了40例良性附件肿块患者；其中25例行腹腔镜手术，15例行开腹手术。术前及术后4小时、24小时和48小时采用市售酶联免疫吸附测定法（ELISA）检测血清中IL-1β、IL-6、IL-8、TNF-α和IL-10的浓度。
两组患者术后4小时、24小时和48小时IL-6浓度均显著升高；术后4小时和24小时IL-10水平显著升高；仅开腹手术后观察到IL-1β水平升高；IL-8血清水平未观察到显著变化；开腹手术患者术后IL-1β、IL-6和IL-10水平的升高比腹腔镜手术患者更明显；手术时间、二氧化碳用量、肿瘤直径、患者年龄和体重指数（BMI）均不影响所研究细胞因子的术后变化模式。
良性附件肿块手术后的全身细胞因子反应取决于手术创伤程度，在腹腔镜手术患者中反应较轻。
