Kumarasamy Yashodharan, Byres Maureen, Cox Philip J, Jaspars Marcel, Nahar Lutfun, Sarker Satyajit D
School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen AB10 1FR, Scotland, UK.
Phytother Res. 2007 Jul;21(7):615-21. doi: 10.1002/ptr.2129.
From a consideration of ethnobotanical and taxonomic information, seeds of 45 Scottish plant species encompassing 23 different families were obtained from authentic seed suppliers. The n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts were assessed, both qualitatively and quantitatively, for free radical scavenging activity in the DPPH assay. The MeOH extracts of 37 species exhibited low to high levels of free radical scavenging activity (RC50 values ranging from 2.00 to 4.7 x 10(-4) mg/mL), and Alliaria petiolata, Prunus padus and Prunus spinosa were the most potent antioxidant extracts. The DCM extracts of 17 species showed similar levels of activity, and among those, Prunus padus and Prunus spinosa extracts were the most active with RC50 values of 2.5 x 10(-4) and 5.0 x 10(-4) mg/mL, respectively. The n-hexane extracts were much less active than the MeOH and DCM extracts, and 17 species, with the exception of Glechoma hederacea (RC50 = 1.94 x 10(-4)) displayed low to moderate levels of free radical scavenging property (RC50 values ranging from 2.00 to 8.7 x 10(-3) mg/mL).
基于民族植物学和分类学信息的考量，从可靠的种子供应商处获取了涵盖23个不同科的45种苏格兰植物的种子。对正己烷、二氯甲烷（DCM）和甲醇（MeOH）提取物进行了DPPH法自由基清除活性的定性和定量评估。37种植物的甲醇提取物表现出低到高的自由基清除活性（RC50值范围为2.00至4.7×10⁻⁴mg/mL），其中，欧洲蒜芥、稠李和黑刺李是最有效的抗氧化提取物。17种植物的二氯甲烷提取物显示出相似的活性水平，其中，稠李和黑刺李提取物活性最高，RC50值分别为2.5×10⁻⁴和5.0×10⁻⁴mg/mL。正己烷提取物的活性远低于甲醇和二氯甲烷提取物，除了连钱草（RC50 = 1.94×10⁻⁴）外，17种植物表现出低到中等水平的自由基清除特性（RC50值范围为2.00至8.7×10⁻³mg/mL）。
