Tokhadze E V, Kvachadze L L, Kvesitadze G I
Prikl Biokhim Mikrobiol. 1975 Jul-Aug;11(4):515-8.
The capacity of 86 strains of the Aspergillus fungus to synthesize acid stable alpha-amylase was examined. The strains of Asp. niger showing a high capacity of synthesizing the enzyme were isolated. Repeated cultivation of the selected cultures on the Minoda agar medium led to a 200% increase in the enzyme activity in the submerged culture. Addition of sodium nitrate to the Minoda medium during submerged cultivation allowed a 3-fold increase of the synthesis of acid stable alpha-amylase.
对86株曲霉菌合成酸稳定α-淀粉酶的能力进行了检测。分离出了黑曲霉中显示出高酶合成能力的菌株。在米诺达琼脂培养基上对所选培养物进行反复培养,使深层培养中的酶活性提高了200%。在深层培养期间向米诺达培养基中添加硝酸钠,可使酸稳定α-淀粉酶的合成增加3倍。