Gerna G, Gullino M, Gerna M T, Achilli G
G Batteriol Virol Immunol. 1975 Jan-Jun;68(1-6):50-64.
Differential ultrastructural features in the replicative cycle between a strain of human cytomegalovirus (CMV) and two strains (types 1 and 2) of herpes simplex virus (HSV) have been studied by electron microscopy. Either on the same or different host cell system four differential aspects have been constantly observed: a) presence in CMV infected cells of a large nuclear inclusion, lacking in HSV infected cells; b) envelopment of CMV capsids by microvescicular systems near the nuclear membrane, whereas HSV (types 1 and 2) takes envelope from the inner lamina of the nuclear membrane; c) presence in the cytoplasm of CMV infected cells of homogeneous dense material and dense microbodies, lacking in HSV infected cells; d) in extracellular space homogenous dense microbodies are present only in CMV infected cell cultures. Diagnostic usefulness of the above reported ultrastructural differences and their meaning on the basis of most recent data of the literature are discussed, with special concern to envelopment of two viruses and to the nature of homogeneous dense material observed in CMV infected cells.
通过电子显微镜研究了人类巨细胞病毒（CMV）的一个毒株与单纯疱疹病毒（HSV）的两个毒株（1型和2型）在复制周期中的超微结构差异特征。在相同或不同的宿主细胞系统上，始终观察到四个不同的方面：a）CMV感染细胞中存在大的核内包涵体，而HSV感染细胞中没有；b）CMV衣壳在核膜附近被微泡系统包裹，而HSV（1型和2型）从核膜的内层获取包膜；c）CMV感染细胞的细胞质中存在均匀的致密物质和致密微体，而HSV感染细胞中没有；d）在细胞外空间中，均匀的致密微体仅存在于CMV感染的细胞培养物中。讨论了上述报道的超微结构差异的诊断用途及其基于文献最新数据的意义，特别关注两种病毒的包膜以及在CMV感染细胞中观察到的均匀致密物质的性质。
