Electron microscopic observations of FL cells infected with herpes simplex virus. III. Dense bodies.

FL cells infected with the -GCr Miyama strain of herpes simplex virus at an adsorbed multiplicity of approximately 10 were fixed at late stages of infection and examined by electron microscopy. Dense bodies containing electron-dense material and surrounded by a limiting membrane were occasionally observed in the perinuclear disternae and in intranuclear vacuoles. Budding of electron-dense material to the cisternae with acquisition of a limiting membrane at the inner nuclear membrane was also occasionally observed. These findings are in constrast with observations that in cells infected with cytomegalovirus numerous dense bodies and their budding process were observed only in the cytoplasmic area.