Ruljancic N, Popovic-Grle S, Rumenjak V, Sokolic B, Malic A, Mihanovic M, Cepelak I
Clinical Laboratory, Sveti Ivan Psychiatric Hospital, Susedgrad, Croatia.
COPD. 2007 Mar;4(1):41-7. doi: 10.1080/15412550601169513.
Magnesium is one of the most important factors for regulation of inflammatory response as well as muscle function, and COPD is a multicomponent disease characterized by abnormal inflammatory response of the lungs with systemic muscle dysfunction. Because polymorphonuclear (PMN) cells are significantly represented in the pathogenesis of COPD, concentrations of total (tMg) and ionised magnesium (iMg) were determined in plasma and isolated PMN cells in 46 patients in stable phase of COPD (past smokers, current smokers, and non-smokers), 24 healthy smokers and 37 healthy non-smokers. In the same samples concentrations of total (tCa) and ionised calcium (iCa) were determined, due to the antagonism of magnesium towards calcium. We found decreased biological active iMg in PMN compared to the group of healthy non-smokers (5.42, 1.98-17.31 micromol/10(9) cells vs. 7.50, 3.27-15.15 micromol/10(9) cells, p < 0.05). In the plasma and isolated PMN of the patients the ratio of total calcium/total magnesium (tCa/tMg) was significantly increased (2.89, 2.15-3.86 and 1.19, 0.07-9.87) compared to the group of healthy non-smokers (2.65, 2.19-3.44 and 0.67, 0.14-2.40, p < 0.05) and to the group of healthy smokers (2.58, 2.26-3.24 and 0.66, 0.14-2.85, p < 0.05). In the group of patients the concentration of tCa was significantly increased in all samples compared to the healthy group of non-smokers and healthy smokers. The results of univariant logistic regression analysis for smoking, concentration of tCa and ratio of tCa/tMg in PMN showed high odds ratio for COPD status. These results raise a possibility that intracellular polymorphonuclear value of magnesium could be a distinctive marker for COPD risk disclosure among smokers.
镁是调节炎症反应以及肌肉功能的最重要因素之一，而慢性阻塞性肺疾病（COPD）是一种多因素疾病，其特征为肺部异常炎症反应并伴有全身肌肉功能障碍。由于多形核（PMN）细胞在COPD发病机制中占显著比例，因此测定了46例COPD稳定期患者（既往吸烟者、当前吸烟者和非吸烟者）、24例健康吸烟者和37例健康非吸烟者血浆及分离出的PMN细胞中的总镁（tMg）和离子化镁（iMg）浓度。由于镁与钙存在拮抗作用，因此在相同样本中还测定了总钙（tCa）和离子化钙（iCa）的浓度。我们发现，与健康非吸烟者组相比，PMN中生物活性iMg降低（5.42，1.98 - 17.31微摩尔/10⁹细胞对7.50，3.27 - 15.15微摩尔/10⁹细胞，p < 0.05）。与健康非吸烟者组（2.65，2.19 - 3.44和0.67，0.14 - 2.40，p < 0.05）以及健康吸烟者组（2.58，2.26 - 3.24和0.66，0.14 - 2.85，p < 0.05）相比，患者血浆及分离出的PMN中总钙/总镁比值（tCa/tMg）显著升高（2.89，2.15 - 3.86和1.19，0.07 - 9.87）。与健康非吸烟者组和健康吸烟者组相比，患者组所有样本中的tCa浓度均显著升高。对吸烟、tCa浓度及PMN中tCa/tMg比值进行的单变量逻辑回归分析结果显示，COPD状态的优势比很高。这些结果增加了一种可能性，即细胞内多形核镁值可能是吸烟者中COPD风险披露的一个独特标志物。
