Higuchi Akihiro, Ueno Ryuji, Shimmura Shigeto, Suematsu Makoto, Dogru Murat, Tsubota Kazuo
Department of Ophthalmology, Keio University School of Medicine, 35 Shinano-machi, Shinjyuku-ku, Tokyo 160-8582, Japan.
Curr Eye Res. 2007 Feb;32(2):83-8. doi: 10.1080/02713680601147690.
Because autologous serum is useful for the treatment of severe dry eye, serum components may be a potential candidate for the treatment of dry eye. Serum albumin is abundantly contained in human serum and plays many physiologic roles. We investigated the efficacy of serum albumin in a dry eye animal model.
Sprague-Dawley rats were used to make dry eye model rats according to a previous study. The central region of the corneal epithelium was scraped mechanically, and the rats were placed in a desiccation room (temperature, 23 +/- 2 degrees C; humidity, 28 +/- 2%; air flow, 2-4 m/s) for 12 hr. During desiccation, one eye of each rat was treated with human serum albumin eye drops, and the other eye was given a drop of phosphate buffered saline (PBS). Human corneal and conjunctival cell lines were used to investigate suppression effect of albumin on apoptosis induced by addition of apoptosis inducers or serum deprivation, respectively.
The erosion area was increased by 12 hr of desiccation. Albumin treatment decreased the area of erosion compared with PBS treatment. Apoptosis suppression assay using cell lines revealed that caspase-3 activation induced by serum deprivation and DNA fragmentation induced by addition of apoptosis inducers were dose-dependently suppressed by albumin.
Albumin showed a therapeutic effect in dry eye model rats. This efficacy may be related to the suppression of apoptosis by albumin.
由于自体血清对严重干眼症的治疗有用，血清成分可能是治疗干眼症的潜在候选物。血清白蛋白大量存在于人体血清中，并发挥多种生理作用。我们研究了血清白蛋白在干眼症动物模型中的疗效。
根据先前的研究，使用Sprague-Dawley大鼠制作干眼症模型大鼠。机械刮除角膜上皮的中央区域，并将大鼠置于干燥室（温度，23±2℃；湿度，28±2%；气流，2-4米/秒）中12小时。在干燥过程中，每只大鼠的一只眼睛用人体血清白蛋白滴眼液治疗，另一只眼睛滴一滴磷酸盐缓冲盐水（PBS）。分别使用人角膜和结膜细胞系研究白蛋白对凋亡诱导剂添加或血清剥夺诱导的细胞凋亡的抑制作用。
干燥12小时后糜烂面积增加。与PBS治疗相比，白蛋白治疗减少了糜烂面积。使用细胞系的凋亡抑制试验表明，血清剥夺诱导的caspase-3激活和凋亡诱导剂添加诱导的DNA片段化被白蛋白剂量依赖性抑制。
白蛋白在干眼症模型大鼠中显示出治疗效果。这种疗效可能与白蛋白对细胞凋亡的抑制有关。
