Schaller J P, Olsen R G
Infect Immun. 1975 Dec;12(6):1405-10. doi: 10.1128/iai.12.6.1405-1410.1975.
A microneutralization assay was developed for antibody-to-subgroup-specific feline oncornaviruses. This study combines the economic advantage of a microtiter system and the quantitative focus reduction method which permits contruction of multiplicity curves for determination of virus-neutralizing titers. A twofold increase in Synder-Theilen feline sarcoma virus (ST-FeSV) on feline embryo cells decreased by approximately twofold the titer of reference goat serum prepared against Kawakami-Theilen feline leukemia virus. Similar dose effects with FeLV serotype virus preparations were not observed. An assay system utilizing FeLV serotypes on sarcoma-positive leukemia-negative cells demonstrated slightly greater sensitivity than one employing ST-FeSV on FE cells. Differential antibody responses to the three subgroup-specific feline oncornaviruses (A, B ,and C) were observed in reference goat sera. This test demonstrated good reproducibility as well as sensitivity and constitutes a significant improvement over end point dilution assay systems.
开发了一种针对猫科肿瘤病毒亚群特异性抗体的微量中和试验。本研究结合了微量滴定系统的经济优势和定量病灶减少方法，该方法允许构建用于测定病毒中和滴度的复数曲线。猫胚胎细胞上的辛德-泰伦猫肉瘤病毒（ST-FeSV）增加两倍，使针对川上-泰伦猫白血病病毒制备的参考山羊血清滴度降低约两倍。未观察到FeLV血清型病毒制剂有类似的剂量效应。在肉瘤阳性白血病阴性细胞上使用FeLV血清型的检测系统显示出比在FE细胞上使用ST-FeSV的检测系统略高的灵敏度。在参考山羊血清中观察到对三种亚群特异性猫科肿瘤病毒（A、B和C）的不同抗体反应。该试验具有良好的重复性和灵敏度，相对于终点稀释检测系统有显著改进。
