• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从大量组织培养液中纯化传染性猫白血病病毒。

Purification of infectious feline leukemia virus from large volumes of tissue culture fluids.

作者信息

Mathes L E, Yohn D S, Olsen R G

出版信息

J Clin Microbiol. 1977 Mar;5(3):372-4. doi: 10.1128/jcm.5.3.372-374.1977.

DOI:10.1128/jcm.5.3.372-374.1977
PMID:192759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274600/
Abstract

Feline leukemia virus from tissue culture fluids was concentrated 100 times by continuous-flow molecular filtration. Virus concentrates retained 100% of their original infectivity. Further purification was accomplished by a single sucrose density gradient centrifugation.

摘要

通过连续流动分子过滤将组织培养液中的猫白血病病毒浓缩100倍。病毒浓缩液保留了其原始感染力的100%。进一步的纯化通过一次蔗糖密度梯度离心完成。

相似文献

1
Purification of infectious feline leukemia virus from large volumes of tissue culture fluids.从大量组织培养液中纯化传染性猫白血病病毒。
J Clin Microbiol. 1977 Mar;5(3):372-4. doi: 10.1128/jcm.5.3.372-374.1977.
2
Theta particles: a structure found in hamster sarcoma virus.θ粒子：一种在仓鼠肉瘤病毒中发现的结构。
J Virol. 1972 Sep;10(3):477-83. doi: 10.1128/JVI.10.3.477-483.1972.
3
Comparisons of virus-like particles from leukotic cattle to feline leukosis virus.患白血病牛的病毒样颗粒与猫白血病病毒的比较。
Bibl Haematol. 1970(36):471-5. doi: 10.1159/000391741.
4
Feline leukemia characterization of the virus.
Bibl Haematol. 1970(36):379-86.
5
Recovery of murine leukemia virus from large volumes of freshly harvested culture fluids by using a single density gradient.通过使用单一密度梯度从大量新鲜收获的培养液中回收鼠白血病病毒。
Appl Microbiol. 1975 Jan;29(1):102-5. doi: 10.1128/am.29.1.102-105.1975.
6
Concentration and purification of poliovirus by ultrafiltration and isopycnic centrifugation.通过超滤和等密度离心法浓缩和纯化脊髓灰质炎病毒。
Appl Microbiol. 1972 Jul;24(1):13-7. doi: 10.1128/am.24.1.13-17.1972.
7
Purification and protein composition of endogenous rat viruses.内源性大鼠病毒的纯化及蛋白质组成
Neoplasma. 1984;31(6):631-40.
8
Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation.
Can J Microbiol. 1980 Nov;26(11):1334-9. doi: 10.1139/m80-221.
9
Isolation of feline leukemia virus from a leopard cat cell line and search for retrovirus in wild felidae.从豹猫细胞系中分离猫白血病病毒并在野生猫科动物中寻找逆转录病毒。
J Natl Cancer Inst. 1981 Oct;67(4):929-33.
10
Virus isolation test for feline leukemia virus.猫白血病病毒的病毒分离试验
J Natl Cancer Inst. 1977 Jan;58(1):129-31. doi: 10.1093/jnci/58.1.129.

引用本文的文献

1
Membrane ultrafiltration: concentration of interleukin-3.膜超滤：白细胞介素-3的浓缩
Appl Environ Microbiol. 1984 May;47(5):1023-6. doi: 10.1128/aem.47.5.1023-1026.1984.
2
Concentration of poliovirus in water by molecular filtration.通过分子过滤法测定水中脊髓灰质炎病毒的浓度
Appl Environ Microbiol. 1980 Aug;40(2):426-8. doi: 10.1128/aem.40.2.426-428.1980.
3
Inhibition of phorbol ester-induced neutrophil chemiluminescence by FeLV.
Arch Virol. 1990;111(1-2):75-85. doi: 10.1007/BF01310506.
4
Monoclonal antibody analysis of neutralization and antibody-dependent enhancement of feline infectious peritonitis virus.猫传染性腹膜炎病毒中和作用及抗体依赖性增强作用的单克隆抗体分析
J Virol. 1992 Nov;66(11):6695-705. doi: 10.1128/JVI.66.11.6695-6705.1992.
5
Induction of endogenous guinea pig retrovirus by 5-bromodeoxyuridine: amplification of virus-specific RNA.5-溴脱氧尿苷诱导内源性豚鼠逆转录病毒：病毒特异性RNA的扩增
J Virol. 1978 Jun;26(3):603-14. doi: 10.1128/JVI.26.3.603-614.1978.
6
Murine neurotropic retrovirus spongiform polioencephalomyelopathy: acceleration of disease by virus inoculum concentration.鼠嗜神经性逆转录病毒海绵状脑脊髓灰质炎：病毒接种物浓度对疾病的加速作用
Infect Immun. 1979 Feb;23(2):540-4. doi: 10.1128/iai.23.2.540-544.1979.

本文引用的文献

1
Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations.从肉瘤37中提取的一种淋巴白血病病毒的生物学研究。I. 起源与初步研究。
J Natl Cancer Inst. 1960 Apr;24:933-51.
2
The isolation of plant viruses by means of "simple" coacervates.利用“简单”凝聚层分离植物病毒
Virology. 1966 Nov;30(3):341-7. doi: 10.1016/0042-6822(66)90112-7.
3
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
4
Purification of oncornaviruses by agglutination with concanacalin A (murine leukemia virus-phytohemagglutinin-friend virus).通过与伴刀豆球蛋白A（鼠白血病病毒-植物血凝素-弗氏病毒）凝集来纯化肿瘤病毒
Proc Natl Acad Sci U S A. 1973 May;70(5):1308-12. doi: 10.1073/pnas.70.5.1308.
5
Production and purification of large amounts of Rous sarcoma virus.大量劳斯肉瘤病毒的生产与纯化。
Appl Microbiol. 1973 Mar;25(3):346-53. doi: 10.1128/am.25.3.346-353.1973.
6
Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".通过聚乙二醇“沉淀法”对水疱性口炎病毒进行浓缩和纯化。
Virology. 1970 Mar;40(3):745-6. doi: 10.1016/0042-6822(70)90219-9.
7
The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid.劳氏肉瘤病毒布莱恩株的核酸：病毒的纯化及核酸的分离
Proc Natl Acad Sci U S A. 1965 Jul;54(1):137-44. doi: 10.1073/pnas.54.1.137.
8
Properties of mouse leukemia viruses. VII. The major viral glycoprotein of friend leukemia virus. Isolation and physicochemical properties.小鼠白血病病毒的特性。VII. 弗氏白血病病毒的主要病毒糖蛋白。分离及物理化学性质。
Virology. 1974 Sep;61(1):100-11. doi: 10.1016/0042-6822(74)90245-1.
9
[Partial purification and biological-serological characterization of carbohydrate containing components from preparations of Friend-leukemia-virus (author's transl)].弗瑞德白血病病毒制剂中含碳水化合物成分的部分纯化及生物学 - 血清学特性（作者译）
Z Naturforsch C. 1973 Nov-Dec;28(11):785-8.
10
Determination of subgroup-specific feline oncornavirus-neutralizing antibody.亚组特异性猫肿瘤病毒中和抗体的测定
Infect Immun. 1975 Dec;12(6):1405-10. doi: 10.1128/iai.12.6.1405-1410.1975.