在突尼斯北部农村地区引起人类和牛cryptosporidiosis 的微小隐孢子虫糖蛋白 60 多样性。
Glycoprotein 60 diversity in Cryptosporidium parvum causing human and cattle cryptosporidiosis in the rural region of Northern Tunisia.
机构信息
Laboratoire de Parasitologie-Mycologie, Institut Pasteur de Tunis, Tunis, Tunisia.
出版信息
Am J Trop Med Hyg. 2014 Feb;90(2):346-50. doi: 10.4269/ajtmh.13-0522. Epub 2013 Dec 16.
The zoonotic potential of Cryptosporidium parvum was studied in an extensive cattle farming region of northern Tunisia. Seventy fecal samples from pre-weaning calves and 403 fecal samples from children were examined by microscopy after modified Ziehl-Neelsen (MZN) staining. Positive Cryptosporidium specimens were identified at a species level using an 18S rRNA nested polymerase chain reaction (PCR) followed by an Restriction Fragment Length Polymorphism (RFLP) analysis. C. parvum isolates were subgenotyped by sequence analysis of the glycoprotein 60 (gp60) gene. Among calf samples, 14 samples were positive by MZN method. C. parvum was identified in all cases. Twelve parvum isolates (85.7%) belonged to family subtype IIa. Subtype IIaA15G2R1 was more prevalent (50%). Two C. parvum isolates corresponded to the IIdA16G1 subtype. Seven human samples were positive by MZN method. C. parvum and C. meleagridis were identified in four and three cases, respectively. Intraspecific characterization of C. parvum identified two subtypes, the IIaA15G2R1 and the IIdA16G1, also found in calves.
在突尼斯北部一个广泛的奶牛养殖区研究了微小隐孢子虫的人畜共患病潜力。通过改良齐尔-尼尔森（MZN）染色，对 70 份犊牛断奶前粪便样本和 403 份儿童粪便样本进行了显微镜检查。使用 18S rRNA 巢式聚合酶链反应（PCR）随后进行限制性片段长度多态性（RFLP）分析，在种水平上鉴定出阳性隐孢子虫标本。通过对糖蛋白 60（gp60）基因的序列分析对微小隐孢子虫分离株进行亚基因型分析。在犊牛样本中，有 14 份样本通过 MZN 方法呈阳性。在所有情况下均鉴定出微小隐孢子虫。12 个微小隐孢子虫分离株（85.7%）属于家族亚型 IIa。亚 IIaA15G2R1 更为普遍（50%）。两个微小隐孢子虫分离株对应于 IIdA16G1 亚型。通过 MZN 方法有 7 个人类样本呈阳性。在 4 例和 3 例中分别鉴定出微小隐孢子虫和微小隐孢子虫/火鸡隐孢子虫。微小隐孢子虫的种内特征鉴定出两个亚型，即 IIaA15G2R1 和 IIdA16G1，也在犊牛中发现。
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