Choi Eun-Mi
Department of Food and Nutrition, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Cell Biol Int. 2007 Sep;31(9):870-7. doi: 10.1016/j.cellbi.2007.01.038. Epub 2007 Feb 11.
The effects of luteolin on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts were investigated. Luteolin (1microM) caused a significant elevation of collagen content, alkaline phosphatase (ALP) activity, and osteocalcin secretion in the cells (P<0.05). The effect of luteolin in increasing collagen content and ALP activity was completely prevented by the presence of 10(-6)M cycloheximide and 10(-6)M tamoxifen, suggesting that luteolin's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of luteolin on the 3-morpholinosydnonimine (SIN-1)-induced production of oxidative stress markers [nitric oxide (NO) and prostaglan E(2) (PGE(2))] and cytokines [tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6)] in osteoblasts. Luteolin (1 and 10microM) decreased the SIN-1-induced production of NO, PGE(2), TNF-alpha, and IL-6 in osteoblasts. These results suggest that inflammatory mediators can be regulated by luteolin stimulating osteoblastic function.
研究了木犀草素对成骨细胞MC3T3-E1细胞功能及成骨细胞中局部因子产生的影响。木犀草素（1微摩尔）使细胞中的胶原蛋白含量、碱性磷酸酶（ALP）活性和骨钙素分泌显著升高（P<0.05）。10^(-6)M放线菌酮和10^(-6)M他莫昔芬的存在完全阻止了木犀草素增加胶原蛋白含量和ALP活性的作用，这表明木犀草素的作用源于新合成的蛋白质成分，并且可能部分参与雌激素作用。然后，我们研究了木犀草素对3-吗啉代辛二亚胺（SIN-1）诱导的成骨细胞氧化应激标志物[一氧化氮（NO）和前列腺素E2（PGE2）]及细胞因子[肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）]产生的影响。木犀草素（1和10微摩尔）降低了SIN-1诱导的成骨细胞中NO、PGE2、TNF-α和IL-6的产生。这些结果表明，木犀草素通过刺激成骨细胞功能可以调节炎症介质。
