Department of Orthodontics, Faculty of Dentistry, Istanbul Aydin University, 34295, Florya, Istanbul, Turkey.
Discipline of Orthodontics and Paediatric Dentistry, School of Dentistry, The University of Sydney, Sydney Dental Hospital, Surry Hills, Australia.
J Orofac Orthop. 2022 Oct;83(Suppl 1):85-95. doi: 10.1007/s00056-021-00366-3. Epub 2022 Jan 11.
The aims of this study were to evaluate the effects of systemic administration of Salvia officinalis Linnaeus (L.) leaf extract on new bone formation in the expanded premaxillary suture in rats in vivo and to examine the antioxidant effects and phenolic profile of Salvia officinalis (SO) leaf and root extracts in vitro.
Fourteen male Sprague Dawley rats were allocated to two groups: SO group (n = 7) and control group (n = 7). An open-loop spring was attached to the upper incisors of each rat to expand the premaxillae. A 5-day expansion period followed by a 12-day retention period was observed. The rats in the SO group received systemic administration of 20 mg SO/kg/day via the orogastric route for 17 days. Histomorphometric examinations were carried out to examine the amount of new bone formation, number of capillaries, and intensity of inflammatory cell response. Immunohistochemical analysis was conducted to examine the number of osteoblasts and osteoclasts. Leaf and root extracts of SO were also analyzed for antioxidant activity and phenolic compounds in vitro.
Statistical analysis showed that the following were higher in the SO group than in the control group: new bone formation, number of osteoblasts and osteoclasts, intensity of inflammatory cell response (neutrophils, lymphocytes, and macrophages), and number of capillaries. The major compound identified in SO leaf extract was rosmarinic acid, while luteolin derivatives, salvianolic acid F, and medioresinol were also present.
Salvia officinalis L. from leaf extract provided antioxidant effects and stimulated enhanced new bone formation in the expanded midpalatal suture after maxillary expansion in rats.
本研究旨在评估体内给予鼠尾草叶提取物对大鼠上颌扩张后扩张前颌缝中新骨形成的影响，并体外研究鼠尾草（SO）叶和根提取物的抗氧化作用和酚类成分。
将 14 只雄性 Sprague Dawley 大鼠随机分为两组：SO 组（n=7）和对照组（n=7）。在每只大鼠的上颌切牙上安装一个开环弹簧以扩张上颌。观察 5 天的扩张期和 12 天的保持期。SO 组大鼠通过口服途径给予 20mg SO/kg/天，共 17 天。进行组织形态计量学检查以检查新骨形成量、毛细血管数量和炎症细胞反应强度。进行免疫组织化学分析以检查成骨细胞和破骨细胞的数量。还对 SO 的叶和根提取物进行了体外抗氧化活性和酚类化合物分析。
统计分析表明，SO 组的以下指标均高于对照组：新骨形成量、成骨细胞和破骨细胞数量、炎症细胞反应强度（中性粒细胞、淋巴细胞和巨噬细胞）和毛细血管数量。SO 叶提取物中的主要化合物是迷迭香酸，同时还存在木犀草素衍生物、丹酚酸 F 和间苯三酚。
鼠尾草叶提取物具有抗氧化作用，并刺激大鼠上颌扩张后扩张的中腭缝中新骨形成增加。
