Rutherfurd S M, Chung T K, Moughan P J
Institute of Food, Nutrition and Human Health, Palmerston North, New Zealand.
Poult Sci. 2007 Apr;86(4):665-72. doi: 10.1093/ps/86.4.665.
The effect of a commercial enzyme preparation containing xylanase, alpha-amylase, and beta-glucanase on dietary AME content and the apparent and true ileal amino acid digestibility of a corn-soy broiler diet and endogenous ileal lysine flow was determined. Two predominantly corn-soy diets also containing wheat bran and canola meal were formulated; one diet contained no added enzymes, whereas the other was supplemented with alpha-amylase, beta-glucanase, and xylanase. Titanium dioxide was included as an indigestible marker. The diets were given to broiler chickens, and AME and true ileal amino acid digestibility were determined. Portions of the 2 test diets were guanidinated and fed to similar aged broiler chickens and endogenous lysine flows determined. The chickens appeared healthy throughout the study, and the mean bird weights at the time of slaughter were not significantly different (P < 0.05) among any of the treatment groups. Dietary AME content was significantly (P < 0.05) higher for the enzyme-supplemented corn-soy diet (2,829 kcal/kg) compared with its unsupplemented control diet (2,766 kcal/kg). True ileal amino acid digestibility was significantly (P < 0.05) higher for all amino acids investigated. The increase ranged from 4% for arginine and glutamic acid to 12% for cystine. There was no significant difference in endogenous ileal lysine flow between broilers fed the unsupplemented diet and those fed the enzyme-supplemented diet. Overall, enzyme supplementation with an enzyme blend containing alpha-amylase, beta-glucanase, and xylanase increased the AME content of a corn-soy broiler diet as well as apparent and true ileal amino acid digestibility for all amino acids, but had no effect on endogenous ileal lysine flow.
测定了一种含有木聚糖酶、α-淀粉酶和β-葡聚糖酶的商业酶制剂对玉米-豆粕型肉鸡日粮的表观代谢能(AME)含量、氨基酸表观和真回肠消化率以及内源性回肠赖氨酸流量的影响。配制了两种主要为玉米-豆粕型且含有麦麸和菜粕的日粮;一种日粮不添加酶,而另一种日粮添加了α-淀粉酶、β-葡聚糖酶和木聚糖酶。添加二氧化钛作为不可消化标记物。将日粮饲喂给肉鸡,测定AME和氨基酸真回肠消化率。将两种试验日粮的部分进行胍基化处理后饲喂给年龄相近的肉鸡,并测定内源性赖氨酸流量。在整个研究过程中,鸡只看起来健康,各处理组之间屠宰时的平均体重无显著差异(P<0.05)。与未添加酶的对照日粮(2,766千卡/千克)相比,添加酶的玉米-豆粕型日粮的日粮AME含量显著更高(P<0.05)(2,829千卡/千克)。所有研究的氨基酸的真回肠消化率均显著更高(P<0.05)。增幅从精氨酸和谷氨酸的4%到胱氨酸的12%不等。饲喂未添加日粮的肉鸡和饲喂添加酶日粮的肉鸡之间的内源性回肠赖氨酸流量无显著差异。总体而言,添加含有α-淀粉酶、β-葡聚糖酶和木聚糖酶的酶制剂混合物可提高玉米-豆粕型肉鸡日粮的AME含量以及所有氨基酸的表观和真回肠消化率,但对内源性回肠赖氨酸流量无影响。