Finne G, Matches J R
J Bacteriol. 1976 Jan;125(1):211-9. doi: 10.1128/jb.125.1.211-219.1976.
Spin-labeling studies were conducted to elucidate the viscosity and phase transition temperatures of lipids isolated from psychrophilic, psychrotrophic, and mesophilic clostridia. Electron spin resonance spectroscopy indicated that the lipids, for all the growth temperatures tested, were in a fluid state and from 13 to 24 C higher than the corresponding lipid transition temperatures. When the organisms were grown at different temperatures, a psychrotropic and two mesophilic clostridia were shown to be able to adjust their lipid-phase transition temperature to the growth temperature. A psychrophilic Clostridium strain, when grown at different temperatures, synthesized lipids that had the same phase transition temperature. It is suggested that this lack of growth temperature-inducible regulation of lipid-phase transition temperature may be a molecular determinant for the psychrophily of this organism. It is proposed that the growth temperature range of an organism is dependent upon the ability of the organism to regulate its lipid fluidity within a specific range.
进行自旋标记研究以阐明从嗜冷、嗜冷兼嗜温及嗜温梭菌中分离出的脂质的粘度和相变温度。电子自旋共振光谱表明，在所测试的所有生长温度下，脂质均处于流体状态，且比相应的脂质转变温度高13至24摄氏度。当生物体在不同温度下生长时，一株嗜冷兼嗜温梭菌和两株嗜温梭菌显示能够将其脂质相变温度调节至生长温度。一株嗜冷梭菌菌株在不同温度下生长时，合成的脂质具有相同的相变温度。有人认为，这种脂质相变温度缺乏生长温度诱导调节的情况可能是该生物体嗜冷性的分子决定因素。有人提出，生物体的生长温度范围取决于其在特定范围内调节脂质流动性的能力。
