缺乏烟酰胺腺嘌呤二核苷酸特异性谷氨酸脱氢酶的构巢曲霉突变体。
Mutants of Aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase.
作者信息
Kinghorn J R, Pateman J A
出版信息
J Bacteriol. 1976 Jan;125(1):42-7. doi: 10.1128/jb.125.1.42-47.1976.
Abstract
Ten mutants of Aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (NAD-GDH) have been isolated, and their mutations (gdhB1 through gdhB10) have been shown to lie in the gdhB gene. In addition, a temperature-sensitive gdhB mutant (gdhB11) has been isolated. A revertant (designated R-5) of the mutant gdhB1 bears an additional lesion in the gdhB gene and has altered NAD-GDH activity with altered Km values for ammonia or ammonium ions and for alpha-ketoglutarate. These results suggest that gdhB specifies a structural component for NAD-GDH. The growth characteristics of gdhB mutants indicate the routes by which amino acids are utilized as nitrogen and carbon energy sources. The properties are described of the double mutants bearing the mutations gdhB1 and gdhA1 or tamA119, which have low NADP-GDH activity.
摘要
已分离出10株缺乏烟酰胺腺嘌呤二核苷酸特异性谷氨酸脱氢酶（NAD-GDH）的构巢曲霉突变体，并且已证明它们的突变（gdhB1至gdhB10）位于gdhB基因中。此外，还分离出了一个温度敏感型gdhB突变体（gdhB11）。突变体gdhB1的一个回复体（命名为R-5）在gdhB基因中有另外一个损伤，并且其NAD-GDH活性发生了改变，对氨或铵离子以及对α-酮戊二酸的Km值也发生了改变。这些结果表明，gdhB指定了NAD-GDH的一个结构成分。gdhB突变体的生长特性表明了氨基酸作为氮源和碳能源被利用的途径。描述了带有gdhB1和gdhA1或tamA119突变的双突变体的特性，这些双突变体具有低NADP-GDH活性。
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本文引用的文献
1
The genetics of Aspergillus nidulans.构巢曲霉的遗传学
Adv Genet. 1953;5:141-238. doi: 10.1016/s0065-2660(08)60408-3.
2
The use of p-fluorophenylalanine with 'master strains' of Aspergillus nidulans for assigning genes to linkage groups.使用对氟苯丙氨酸与构巢曲霉“标准菌株”将基因定位到连锁群上。
Genet Res. 1965 Nov;6(3):352-9. doi: 10.1017/s0016672300004249.
3
Regulation of synthesis of glutamate dehydrogenase and glutamine synthetase in micro-organisms.微生物中谷氨酸脱氢酶和谷氨酰胺合成酶合成的调控
Biochem J. 1969 Dec;115(4):769-75. doi: 10.1042/bj1150769.
4
Metabolism of threonine by penicillia: growth on threonine as the sole carbon and nitrogen source.
J Gen Microbiol. 1972 Nov;73(1):71-83. doi: 10.1099/00221287-73-1-71.
5
A possible role in the regulation of primary animation for a complex of glutamine: -ketoglutarate amidotransferase and glutamate dehydrogenase in Escherichia coli.
Biochem Biophys Res Commun. 1972 Jul 11;48(1):41-7. doi: 10.1016/0006-291x(72)90341-5.
6
Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of saccharomyces cerevisiae.氨对普通氨基酸通透酶的抑制作用及其在酿酒酵母NADPH特异性谷氨酸脱氢酶缺失突变体中的抑制解除。
Biochem Biophys Res Commun. 1972 Aug 21;48(4):749-56. doi: 10.1016/0006-291x(72)90670-5.
7
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Biochem Biophys Res Commun. 1972 Jun 28;47(6):1498-503. doi: 10.1016/0006-291x(72)90242-2.
8
The regulation of NAD L-glutamate dehydrogenase in Aspergillus nidulans.
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9
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10
The effect of the carbon source on ammonium regulation in Aspergillus nidulans.
Mol Gen Genet. 1974;128(1):95-8. doi: 10.1007/BF00267298.
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