Chen WenChieh, Yang Chao-Chun, Tsai Ren-Yeu, Liao Chen-Yu, Yen Yu-Ta, Hung Chiu-Ling, Chen Ko-Fan, Tsai Shaw-Jenq, Zouboulis Christos C
Department of Dermatology, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC.
Dermatology. 2007;214(3):199-204. doi: 10.1159/000099583.
The regulation of the cutaneous steroidogenesis in patients with androgenetic alopecia remains largely unclear.
The purpose of this study was to quantify the expression of the sex-determining genes in different scalp areas.
Paired scalp specimens from frontal and occipital scalp areas of 10 patients were examined by real-time RT-PCR for mRNA expression and of 40 patients (mean age 34.9 years, range 22-58) by Western blotting for protein analysis.
The SOX-9 mRNA was most abundant in the skin, while SF-1 mRNA was sparsely detected. The protein levels of DAX-1, SRY and WT-1 were significantly higher in the bald scalp (p=0.003, 0.004 and 0.03, respectively). Only the SRY expression showed a positive correlation with the baldness severity in Norwood-Hamilton classification (p=0.024). There was no association between patient's age and the protein levels. Immunostaining of SOX-9 was detected in the outer root sheath keratinocytes of hair follicles but not in the dermal papillae.
Further study on a larger population, including normal subjects and female patients, is needed to confirm the pathogenic role of sex-determining genes in androgenetic alopecia.
雄激素性脱发患者皮肤类固醇生成的调节机制仍不清楚。
本研究旨在量化不同头皮区域性别决定基因的表达。
对10例患者额部和枕部头皮区域的配对头皮标本进行实时逆转录聚合酶链反应检测mRNA表达，对40例患者（平均年龄34.9岁，范围22 - 58岁）进行蛋白质印迹分析蛋白质。
SOX - 9 mRNA在皮肤中含量最高，而SF - 1 mRNA检测较少。DAX - 1、SRY和WT - 1的蛋白质水平在秃发头皮中显著更高（分别为p = 0.003、0.004和0.03）。仅SRY表达与诺伍德 - 汉密尔顿分类中的秃发严重程度呈正相关（p = 0.024）。患者年龄与蛋白质水平之间无关联。在毛囊外根鞘角质形成细胞中检测到SOX - 9免疫染色，但在真皮乳头中未检测到。
需要对包括正常受试者和女性患者在内的更大样本进行进一步研究，以证实性别决定基因在雄激素性脱发中的致病作用。
