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小RNA调控因子与细菌的应激反应

Small RNA regulators and the bacterial response to stress.

作者信息

Gottesman S, McCullen C A, Guillier M, Vanderpool C K, Majdalani N, Benhammou J, Thompson K M, FitzGerald P C, Sowa N A, FitzGerald D J

机构信息

Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

出版信息

Cold Spring Harb Symp Quant Biol. 2006;71:1-11. doi: 10.1101/sqb.2006.71.016.

Abstract

Recent studies have uncovered dozens of regulatory small RNAs in bacteria. A large number of these small RNAs act by pairing to their target mRNAs. The outcome of pairing can be either stimulation or inhibition of translation. Pairing in vivo frequently depends on the RNA-binding protein Hfq. Synthesis of these small RNAs is tightly regulated at the level of transcription; many of the well-studied stress response regulons have now been found to include a regulatory RNA. Expression of the small RNA can help the cell cope with environmental stress by redirecting cellular metabolism, exemplified by RyhB, a small RNA expressed upon iron starvation. Although small RNAs found in Escherichia coli can usually be identified by sequence comparison to closely related enterobacteria, other approaches are necessary to find the equivalent RNAs in other bacterial species. Nonetheless, it is becoming increasingly clear that many if not all bacteria encode significant numbers of these important regulators. Tracing their evolution through bacterial genomes remains a challenge.

摘要

最近的研究在细菌中发现了几十种调控性小RNA。其中大量的小RNA通过与它们的靶标mRNA配对发挥作用。配对的结果可以是刺激或抑制翻译。体内配对通常依赖于RNA结合蛋白Hfq。这些小RNA的合成在转录水平受到严格调控;现在发现许多经过充分研究的应激反应调节子都包含一种调控RNA。小RNA的表达可以通过重定向细胞代谢帮助细胞应对环境应激,以铁饥饿时表达的小RNA RyhB为例。虽然在大肠杆菌中发现的小RNA通常可以通过与密切相关的肠道细菌进行序列比较来鉴定,但在其他细菌物种中找到等效的RNA则需要其他方法。尽管如此,越来越清楚的是,许多(如果不是所有的话)细菌都编码大量这些重要的调节子。通过细菌基因组追踪它们的进化仍然是一个挑战。

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本文引用的文献

1
Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins.
J Mol Biol. 2006 Nov 17;364(1):1-8. doi: 10.1016/j.jmb.2006.09.004. Epub 2006 Sep 8.
3
Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq.
Mol Microbiol. 2006 Aug;61(4):1013-22. doi: 10.1111/j.1365-2958.2006.05288.x. Epub 2006 Jul 12.
4
The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.
BMC Microbiol. 2006 Jun 12;6:52. doi: 10.1186/1471-2180-6-52.
5
Target prediction for small, noncoding RNAs in bacteria.
Nucleic Acids Res. 2006 May 22;34(9):2791-802. doi: 10.1093/nar/gkl356. Print 2006.
6
Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction.
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4858-63. doi: 10.1073/pnas.0509638103. Epub 2006 Mar 20.
8
Hfq-dependent alterations of the transcriptome profile and effects on quorum sensing in Pseudomonas aeruginosa.
Mol Microbiol. 2006 Mar;59(5):1542-58. doi: 10.1111/j.1365-2958.2006.05032.x.
9
Experimental approaches to identify non-coding RNAs.
Nucleic Acids Res. 2006 Jan 25;34(2):635-46. doi: 10.1093/nar/gkj469. Print 2006.
10
Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs.
Mol Microbiol. 2006 Jan;59(1):231-47. doi: 10.1111/j.1365-2958.2005.04929.x.

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