Gottesman Susan
Laboratory of Molecular Biology, NCI, United States.
J Mol Biol. 2025 Jun 1;437(11):169059. doi: 10.1016/j.jmb.2025.169059. Epub 2025 Mar 3.
I was lucky to start my research career as the molecular biology revolution was taking hold, providing a constantly increasing set of tools and questions to investigate. Starting from a fascination with bacteria and their ability to adapt to different conditions, I've investigated post-translational mechanisms and their role in the ability of E. coli to respond to stress. My research career has been primarily at the National Institutes of Health, where I run a group within the Laboratory of Molecular Biology, NCI and hold the title of NIH Distinguished Investigator. Our lab has been interested in both energy-dependent proteolysis, discussed very briefly here, and small regulatory RNAs (sRNAs). The major group of such sRNAs act by pairing with target mRNAs with the aid of the RNA chaperone Hfq, mediating both positive and negative regulation of translation and mRNA stability. Both in our own lab and in a continuing and highly productive collaboration with the laboratory of Gisela (Gigi) Storz, we have used global approaches to identify novel sRNAs, identified how many of them are regulated, both at the level of transcription and stability, and worked on understanding the role of these sRNAs in regulatory networks. Our continued work explores regulators of sRNA and Hfq function. Here, Gigi and I have split summaries of our findings, and hope that our two chapters will be read together.
我很幸运在分子生物学革命蓬勃发展之际开启了我的研究生涯，这为研究提供了越来越多的工具和问题。出于对细菌及其适应不同条件能力的着迷，我研究了翻译后机制及其在大肠杆菌应激反应能力中的作用。我的研究生涯主要是在美国国立卫生研究院度过的，我在国立癌症研究所分子生物学实验室领导一个团队，并拥有美国国立卫生研究院杰出研究员的头衔。我们实验室一直对能量依赖型蛋白水解（在此简要讨论）和小调节RNA（sRNA）感兴趣。这类sRNA的主要群体通过与RNA伴侣Hfq结合，与靶mRNA配对发挥作用，介导翻译和mRNA稳定性的正调控和负调控。无论是在我们自己的实验室，还是在与吉塞拉（吉吉）·施托兹实验室持续且卓有成效的合作中，我们都采用全局方法来鉴定新型sRNA，确定其中有多少在转录和稳定性水平上受到调控，并致力于理解这些sRNA在调控网络中的作用。我们持续的工作探索sRNA和Hfq功能的调节因子。在此，吉吉和我分别总结了我们的发现，希望我们的两章内容能一起阅读。
