胆固醇酯酶和胆固醇氧化酶在聚苯胺薄膜上的共价固定化及其在胆固醇生物传感器中的应用。

Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor.

作者信息

Singh Suman, Solanki Pratima R, Pandey M K, Malhotra B D

机构信息

Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.

出版信息

Anal Chim Acta. 2006 May 24;568(1-2):126-32. doi: 10.1016/j.aca.2005.10.008. Epub 2005 Nov 15.

DOI:10.1016/j.aca.2005.10.008

PMID:17761252

Abstract

Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) have been covalently immobilized on electrochemically prepared polyaniline (PANI) films. These PANI/ChEt/ChOx enzyme films have been characterized using UV-visible, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Electrochemical behavior of these films has been studied using cyclic voltammetry (CV) and amperometric techniques, respectively. The PANI/ChEt/ChOx enzyme films show broad oxidation peak from 0.2 to 0.5 V. These PANI/ChEt/ChOx biosensing electrodes have a response time of about 40s, linearity from 50 to 500 mg/dl of cholesterol oleate concentration. These PANI/ChEt/ChOx films are thermally stable up to 46 degrees C. This polyaniline based cholesterol biosensor has optimum pH in the range of 6.5-7.5, sensitivity as 7.5x10(-4) nA/mg dl and a lifetime of about 6 weeks.

摘要

胆固醇酯酶（ChEt）和胆固醇氧化酶（ChOx）已被共价固定在电化学制备的聚苯胺（PANI）薄膜上。这些聚苯胺/胆固醇酯酶/胆固醇氧化酶酶膜已通过紫外可见光谱、傅里叶变换红外（FTIR）光谱和扫描电子显微镜（SEM）进行了表征。分别使用循环伏安法（CV）和安培技术研究了这些膜的电化学行为。聚苯胺/胆固醇酯酶/胆固醇氧化酶酶膜在0.2至0.5 V范围内显示出宽氧化峰。这些聚苯胺/胆固醇酯酶/胆固醇氧化酶生物传感电极的响应时间约为40秒，油酸胆固醇浓度在50至500 mg/dl范围内呈线性。这些聚苯胺/胆固醇酯酶/胆固醇氧化酶膜在高达46摄氏度时热稳定。这种基于聚苯胺的胆固醇生物传感器的最佳pH值在6.5 - 7.5范围内，灵敏度为7.5×10^(-4) nA/mg dl，寿命约为6周。