用于胆固醇生物传感器的氧化锌纳米颗粒-壳聚糖复合膜

Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor.

作者信息

Khan Raju, Kaushik Ajeet, Solanki Pratima R, Ansari Anees A, Pandey Manoj K, Malhotra B D

机构信息

Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110 012, India.

出版信息

Anal Chim Acta. 2008 Jun 2;616(2):207-13. doi: 10.1016/j.aca.2008.04.010. Epub 2008 Apr 11.

DOI:10.1016/j.aca.2008.04.010

PMID:18482605

Abstract

Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41x10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K(m)) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.

摘要

均匀分散在壳聚糖（CHIT）中的氧化锌纳米颗粒（NanoZnO）已被用于在氧化铟锡（ITO）玻璃板上制备混合纳米复合膜。胆固醇氧化酶（ChOx）已使用物理吸附技术固定在该NanoZnO-CHIT复合膜上。分别使用傅里叶变换红外（FTIR）、X射线衍射（XRD）、循环伏安法（CV）、扫描电子显微镜（SEM）和电化学阻抗谱（EIS）技术对NanoZnO-CHIT/ITO电极和ChOx/NanoZnO-CHIT/ITO生物电极进行了表征。ChOx/NanoZnO-CHIT/ITO生物电极在5至300 mg dl⁻¹的胆固醇浓度范围内呈现线性，检测限为5 mg dl⁻¹，灵敏度为1.41×10⁻⁴ A mg dl⁻¹，米氏常数（Kₘ）值为8.63 mg dl⁻¹。这种胆固醇生物传感器可用于估算血清样本中的胆固醇。