Smith R E, Davids L J, Neiman P E
J Virol. 1975 Jan;17(1):160-7. doi: 10.1128/JVI.17.1.160-167.1976.
Myeloblastosis-associated virus (MAV)-2(0), a virus which was derived from avian myeloblastosis virus and induced a high incidence of osteopetrosis, was compared with avian lymphomatosis virus 5938, a recent field isolate which induced a high incidence of lymphomatosis. The following information was obtained. (i) MAV-2(0) induced osteopetrosis, nephroblastoma, and a very low incidence of hepatocellular carcinoma. No difference was seen in the oncogenic spectrum of end point and plaque-purified MAV-2(0). (ii) 125I-labeled RNA sequences from MAV-2(0) formed hybrids with DNA extracted from osteopetrotic bone at a rate suggesting five proviral copies per haploid cell genome. The extent of hybridization of MAV-2(0) RNA with DNA from osteopetrotic tissue was more extensive (87%) than was observed in reactions with DNA from uninfected chicken embryos (52%). (iii) Competition of unlabeled viral RNA in hybridization reactions between the radioactive RNA from the two viruses and their respective proviral sequences present in tumor tissues showed that 15 to 20% of the viral sequences detected in these reactions were unshared. In contrast, no differences were detected in competition analyses of RNA sequences from the two viruses detected in DNA of normal chicken cells. (iv) MAV-2(0) 35S RNA was indistinguishable in size from avian lymphomatosis virus 5938 35S RNA by polyacrylamide gel electrophoresis.
成髓细胞增多症相关病毒（MAV）-2(0)，一种源自禽成髓细胞增多症病毒并引发高发性骨硬化症的病毒，与禽淋巴瘤病毒5938（一种近期从野外分离出且引发高发性淋巴瘤的病毒）进行了比较。获得了以下信息。（i）MAV-2(0)引发骨硬化症、肾母细胞瘤以及极低发生率的肝细胞癌。终点法和蚀斑纯化的MAV-2(0)在致癌谱方面未见差异。（ii）来自MAV-2(0)的125I标记RNA序列与从骨硬化症骨骼中提取的DNA形成杂交体，其速率表明单倍体细胞基因组中每细胞有五个前病毒拷贝。MAV-2(0) RNA与骨硬化症组织DNA的杂交程度（87%）比与未感染鸡胚DNA反应中观察到的（52%）更广泛。（iii）在两种病毒的放射性RNA与肿瘤组织中各自前病毒序列的杂交反应中，未标记病毒RNA的竞争表明，在这些反应中检测到的病毒序列有15%至20%是不共享的。相比之下，在正常鸡细胞DNA中检测到的两种病毒RNA序列的竞争分析中未检测到差异。（iv）通过聚丙烯酰胺凝胶电泳，MAV-2(0) 35S RNA在大小上与禽淋巴瘤病毒5938 35S RNA无法区分。
