Liu P, Piao X S, Kim S W, Wang L, Shen Y B, Lee H S, Li S Y
China Agricultural University, Ministry of Agriculture Feed Industry Centre, Beijing 100193, China.
J Anim Sci. 2008 Oct;86(10):2609-18. doi: 10.2527/jas.2007-0668. Epub 2008 May 23.
A total of 50 weaning pigs (16 d of age; 4.72 +/- 0.23 kg of BW) were selected to investigate the effect of dietary chito-oligosaccharide (COS) supplementation on growth performance, fecal shedding of Escherichia coli and Lactobacillus, apparent digestibility, and small intestinal morphology. Pigs housed in individual metabolic cages were assigned randomly to 5 treatments (n = 10), including 1 basal diet (control), 3 diets with COS supplementation (100, 200, and 400 mg/kg), and 1 diet with chlortetracycline (CTC) supplementation (80 mg/kg). Fresh fecal samples were collected to evaluate shedding of E. coli and Lactobacillus on d 0, 7, 14, and 21 postweaning. Fresh fecal samples collected from each cage from d 19 to 21 were stored frozen for determination of apparent total tract digestibility. On d 21, all pigs were killed to collect the middle sections of the duodenum, jejunum, and ileum for determination of mucosa morphology. Supplementation of COS at 100 and 200 mg/kg and supplementation of CTC improved (P < 0.05) overall ADG, ADFI, and G:F in comparison with the control. Supplementation of COS at 200 mg/kg as well as supplementation of CTC increased (P < 0.05) apparent total tract digestibility of DM, GE, CP, crude fat, Ca, and P, whereas COS at 100 mg/kg increased (P < 0.05) the digestibility of DM, Ca, and P in comparison with the control diet. Pigs receiving diets supplemented with COS or CTC had a decreased (P < 0.05) incidence of diarrhea and decreased diarrhea scores compared with control pigs. Fecal samples from pigs receiving diets supplemented with COS had greater (P < 0.05) Lactobacillus counts than those from control pigs and pigs receiving diets supplemented with CTC on d 14 and 21. However, supplementation of COS at 200 mg/kg and supplementation of CTC decreased (P < 0.05) E. coli counts in the feces on d 21 compared with the control diet. Dietary supplementation of COS at 200 mg/kg and of CTC increased (P < 0.05) the villus height and villus:crypt ratio at the ileum and jejunum, and COS at 100 mg/kg also increased (P < 0.05) the villus height in the ileum compared with the control diet. The current results indicated that dietary supplementation of COS at 100 and 200 mg/kg enhanced growth performance by increasing apparent digestibility, decreasing the incidence of diarrhea, and improving small intestinal morphology.
选取50头断奶仔猪（16日龄；体重4.72±0.23千克），研究日粮添加壳寡糖（COS）对生长性能、粪便中大肠杆菌和乳酸杆菌排出量、表观消化率及小肠形态的影响。将饲养在个体代谢笼中的仔猪随机分为5组（每组10头），包括1个基础日粮组（对照组）、3个添加COS的日粮组（100、200和400毫克/千克）以及1个添加金霉素（CTC）的日粮组（80毫克/千克）。在断奶后第0、7、14和21天采集新鲜粪便样本，评估大肠杆菌和乳酸杆菌的排出量。从第19天至21天，从每个笼子采集新鲜粪便样本并冷冻保存，用于测定表观全肠道消化率。在第21天，宰杀所有仔猪，采集十二指肠、空肠和回肠的中段，用于测定黏膜形态。与对照组相比，添加100和200毫克/千克COS以及添加CTC可提高（P<0.05）总体平均日增重、平均日采食量和料重比。添加200毫克/千克COS以及添加CTC可提高（P<0.05）干物质、总能、粗蛋白、粗脂肪、钙和磷的表观全肠道消化率，而添加100毫克/千克COS与对照日粮相比，可提高（P<0.05）干物质、钙和磷的消化率。与对照仔猪相比，采食添加COS或CTC日粮的仔猪腹泻发生率降低（P<0.05），腹泻评分降低。在第14天和21天，采食添加COS日粮的仔猪粪便中乳酸杆菌数量高于对照仔猪和采食添加CTC日粮的仔猪（P<0.05）。然而，与对照日粮相比，添加200毫克/千克COS和添加CTC可降低第21天粪便中大肠杆菌数量（P<0.05）。日粮添加200毫克/千克COS和添加CTC可提高（P<0.05）回肠和空肠的绒毛高度及绒毛高度与隐窝深度之比，添加100毫克/千克COS与对照日粮相比，也可提高（P<0.05）回肠的绒毛高度。当前结果表明，日粮添加100和200毫克/千克COS可通过提高表观消化率、降低腹泻发生率和改善小肠形态来提高生长性能。
