Kong X F, Zhang Y Z, Wu X, Yin Y L, Tan Z L, Feng Y, Yan F Y, Bo M J, Huang R L, Li T J
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, China.
Int J Microbiol. 2009;2009:598152. doi: 10.1155/2009/598152. Epub 2009 Aug 25.
Rat was used to characterize Chinese Yam polysaccharides (CYPs). In Exp. 1, maximum volume and rate of gas production in CYP 3-supplemented group were higher than other CYP-supplemented groups and control group, while pH values and NH3 contents in CYP 2-, CYP 3-, and CYP 4-supplemented groups were lower than control group. Contents of acetate, propionate and butyrate increased by supplementing CYP 3 or CYP 4 compared to other groups, except for glucose-supplemented group. Contents of isobutyrate for CYPs groups decreased compared to control group. CYP 3 enhanced beneficial gut microbiota, but suppressed bacterial pathogens. In Exp. 2, contents of acetate and butyrate in cecal digesta of rats fed 0.25 or 0.5 g/kg CYP 3 were higher than other groups on day 7. pH values in 0.25 and 0.5 g/kg groups were lower than 1.0 g/kg group. Contents of acetate in 0.25 and 0.5 g/kg groups were greater than other 2 groups on day 21. Gut microflora in CYP 3-supplemented rats had greater diversity than non-supplemented rats. CYP 3 enriched beneficial gut microbiota, but suppressed bacterial pathogens in rat cecum. These findings suggested that CYP 3 is a good source of carbon and energy, and may improve bacterial community diversity and modulate short-chain fatty acid production in hindgut of rats.
采用大鼠来表征山药多糖（CYPs）。在实验1中，补充CYP 3组的气体产生最大体积和速率高于其他补充CYP组和对照组，而补充CYP 2、CYP 3和CYP 4组的pH值和NH3含量低于对照组。与其他组相比，补充CYP 3或CYP 4后乙酸、丙酸和丁酸的含量增加，但葡萄糖补充组除外。CYPs组的异丁酸含量与对照组相比有所下降。CYP 3增强了有益肠道微生物群，但抑制了细菌病原体。在实验2中，在第7天，喂食0.25或0.5 g/kg CYP 3的大鼠盲肠消化物中乙酸和丁酸的含量高于其他组。0.25和0.5 g/kg组的pH值低于1.0 g/kg组。在第21天，0.25和0.5 g/kg组的乙酸含量高于其他两组。补充CYP 3的大鼠肠道微生物群比未补充的大鼠具有更高的多样性。CYP 3丰富了大鼠盲肠中的有益肠道微生物群，但抑制了细菌病原体。这些发现表明，CYP 3是碳和能量的良好来源，可能改善细菌群落多样性并调节大鼠后肠中短链脂肪酸的产生。
