Bomba Alojz, Nemcová Radomíra, Gancarcíková Sona, Herich Robert, Pistl Juraj, Révajová Viera, Jonecová Zuzana, Bugarský Andrej, Levkut Mikulás, Kasteĺ Rudolf, Baran Miroslav, Lazar Gabriel, Hluchý Marián, Marsálková Slávka, Posivák Ján
University of Veterinary Medicine, Kosice, Slovak Republic.
Berl Munch Tierarztl Wochenschr. 2003 Jul-Aug;116(7-8):312-6.
The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillus paracasei to the jejunal mucosa of gnotobiotic piglets. When compared to the control group, the number of Lactobacillus paracasei adhering to the jejunal mucosa was by 12% higher in piglets of the experimental group (5.10 log 10/cm2 vs. 4.55 log 10/cm2). The respective counts of Lactobacillus paracasei adhering to the ileal and colonic mucosa of 28 day old gnotobiotic piglets reached 4.45 and 5.05 log 10/cm2 in group C and 4.44 and 4.95 log 10/cm2 in group E. Omega-3 PUFA supplementation increased the phagocytic activity of neutrophils by almost 100% on day 28 of life as well as the subpopulations of lymphocytes (CD8) in the peripheral blood of germ-free piglets on day 21 of life. Our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA. The stimulatory effect of PUFA upon adhesion of lactobacilli could be used for enhancing the effectiveness of probiotics in inhibiting digestive tract pathogens.
在无菌猪中研究了ω-3多不饱和脂肪酸（ω-3 PUFA）的应用对副干酪乳杆菌肠道定植及细胞免疫的影响。多不饱和脂肪酸的施用对副干酪乳杆菌黏附于无菌仔猪空肠黏膜有积极影响。与对照组相比，实验组仔猪空肠黏膜上黏附的副干酪乳杆菌数量高出12%（5.10 log 10/cm²对4.55 log 10/cm²）。在28日龄无菌仔猪中，C组黏附于回肠和结肠黏膜的副干酪乳杆菌数量分别达到4.45和5.05 log 10/cm²，E组为4.44和4.95 log 10/cm²。补充ω-3 PUFA可使出生后第28天的中性粒细胞吞噬活性提高近100%，并使无菌仔猪出生后第21天外周血中的淋巴细胞亚群（CD8）增加。我们的结果表明，肠道中益生菌的作用可能受膳食多不饱和脂肪酸的调节。多不饱和脂肪酸对乳酸杆菌黏附的刺激作用可用于提高益生菌抑制消化道病原体的有效性。
