Tang Shuo, Sun Chung-Ho, Krasieva Tatiana B, Chen Zhongping, Tromberg Bruce J
Department of Electrical and Computer Engineering, University of British Columbia, Vancouver V6T 1Z4, Canada.
Opt Lett. 2007 Mar 1;32(5):503-5. doi: 10.1364/ol.32.000503.
The structural origin of scattering contrast from single cells is examined by using a combined optical coherence and multiphoton microscope based on a 12 fs Ti:sapphire source and a 0.95 NA objective. High-resolution coherence-gated scattering images from single cells are coregistered and compared with two-photon-excited fluorescence images. Scattering contrast is observed from mitochondria, plasma membrane, actin filaments, and the boundary between cytoplasm and nucleus. There is little contribution to scattering from regions inside the nuclear core. These results confirm that light scattering signals from specific subcellular structures can be visualized by using coherent reflectance geometry.
利用基于12飞秒钛宝石光源和0.95数值孔径物镜的光学相干和多光子显微镜组合，研究了单细胞散射对比度的结构起源。来自单细胞的高分辨率相干选通散射图像被配准，并与双光子激发荧光图像进行比较。在线粒体、质膜、肌动蛋白丝以及细胞质和细胞核之间的边界处观察到散射对比度。核芯内部区域对散射的贡献很小。这些结果证实，通过使用相干反射几何结构，可以可视化来自特定亚细胞结构的光散射信号。
