Yera H, Zamfir O, Bourcier T, Ancelle T, Batellier L, Dupouy-Camet J, Chaumeil C
Laboratoire de Parasitologie Mycologie, Hôpital Cochin, AP-HP, Université Paris Descartes, 27 rue du Faubourg Saint Jacques, 75679 Paris Cedex 14, France.
Eur J Clin Microbiol Infect Dis. 2007 Mar;26(3):221-4. doi: 10.1007/s10096-007-0268-6.
In the study presented here, PCR, microscopic examination and culture of corneal samples were compared as methods of confirming the clinical diagnosis of Acanthamoeba keratitis, a serious ocular infection that is difficult to diagnose and threatens eyesight. The three methods were applied to isolates obtained from 513 patients with clinical signs or risk factors suggesting Acanthamoeba infection. Acanthamoeba keratitis was diagnosed in 12 of these patients. Combined PCR assays were more sensitive (94%) than either microscopic examination (33%) or culture (7%). The Acanthamoeba isolates were characterized using DNA sequence analysis of the nuclear small-subunit rRNA gene, and T4 was the predominant genotype found.
在本文介绍的研究中，对角膜样本进行聚合酶链反应（PCR）、显微镜检查和培养，以此作为确诊棘阿米巴角膜炎临床诊断的方法进行比较。棘阿米巴角膜炎是一种严重的眼部感染，难以诊断且会威胁视力。这三种方法应用于从513例有临床体征或提示棘阿米巴感染风险因素的患者中分离出的菌株。这些患者中有12例被诊断为棘阿米巴角膜炎。联合PCR检测比显微镜检查（33%）或培养（7%）更敏感（94%）。利用核小亚基rRNA基因的DNA序列分析对棘阿米巴分离株进行特征鉴定，发现T4是主要的基因型。
