Lindgren F T, Adamson G L, Jenson L C, Wood P D
Lipids. 1975 Dec;10(12):750-6. doi: 10.1007/BF02532316.
From a parent population of 774, a subpopulation of 160 normal adults ages 27-66 was randomly selected, 20 from each decade and sex. A detailed comparison was made by analytic ultracentrifugation and complete agarose gel electrophoresis on serum and the 1.006 g/ml top and bottom preparative ultracentrifuge lipoprotein fractions. The latter was internally standardized by total lipid and plasma total cholesterol and triglyceride determinations giving normal reference lipoprotein values. The reading procedure allowed the identification and quantification of floating beta and sinking pre-beta. In the subpopulation, there were two of the former and 13 of the latter. For large scale clinical application of such quantitative lipoprotein electrophoresis full automation of the microdensitometry and calculations will be required.
从774人的总体人群中，随机选取了160名年龄在27至66岁之间的正常成年人作为亚群，每个十年和性别各选20人。通过分析超速离心法以及对血清和1.006 g/ml顶部和底部制备超速离心脂蛋白组分进行完整的琼脂糖凝胶电泳，进行了详细比较。后者通过总脂质、血浆总胆固醇和甘油三酯测定进行内部标准化，得出正常参考脂蛋白值。读取程序可以识别并定量漂浮的β脂蛋白和下沉的前β脂蛋白。在该亚群中，有2例前者和13例后者。对于这种定量脂蛋白电泳的大规模临床应用，微密度测定法和计算将需要完全自动化。
