Myers L H, Phillips N R, Havel R J
J Lab Clin Med. 1976 Sep;88(3):491-505.
The concentration of total cholesterol and triglycerides in the three major lipoprotein classes of human serum was measured in 136 men, randomly selected from an industrial population, by a quantitative method of lipoprotein electrophoresis on agarose gel and of fractions separated by preparative ultracentrifugation. Correlation coefficients for the two estimates were 0.98 for triglycerides in very low-density lipoproteins, 0.93 for total cholesterol in low-density lipoproteins, and 0.75 for total cholesterol in high-density lipoproteins. Data obtained form the analyses of the ultracentrifugal fractions were used to develop regression equations that predict the concentrations of total cholesterol and triglycerides in the lipoprotein classes from their concentrations in whole serum. These equations take into account the inverse curvilinear relationship between total cholesterol in high-density lipoproteins and serum tiriglyceride concentration. When applied to a separate sample of 530 men, the predicted values for triglycerides in very low-density lipoproteins and total cholesterol in low-density lipoproteins correlated as well with ultracentrifugal values as did the electrophoretic estimates. However, for total cholesterol in high-density lipoproteins, the electrophoretic method was superior. Similar regression equations were developed from ultracentrifugal lipoprotein analyses in 158 women from the same industrial population. Although the concentration of total cholesterol in the low-density lipoproteins estimated by both electrophoresis and the regression equations agreed closely in most cases with the ultracentrifugal values, errors exceeded 10% with sufficient frequency to limit the value of the estimates for this purpose. In both men and women, the ratio of total cholesterol to triglycerides in high-density lipoproteins was a hyperbolic function of serum triglyceride concentration, suggesting that cholesteryl esters in the core of this lipoprotein are progressively replaced by triglycerides as the concentration of triglycerides in very low-density lipoproteins increases. This altered composition of nonpolar lipids accounts, at least in part, for the reduction of cholesterol in high-density lipoproteins in hyperlipemic individuals.
采用琼脂糖凝胶脂蛋白电泳定量法和制备性超速离心分离组分的方法，对从工业人群中随机选取的136名男性的人血清中三种主要脂蛋白类别的总胆固醇和甘油三酯浓度进行了测定。两种估算方法的相关系数分别为：极低密度脂蛋白中甘油三酯的相关系数为0.98，低密度脂蛋白中总胆固醇的相关系数为0.93，高密度脂蛋白中总胆固醇的相关系数为0.75。从超速离心组分分析中获得的数据用于建立回归方程，该方程可根据全血清中总胆固醇和甘油三酯的浓度预测脂蛋白类别中它们的浓度。这些方程考虑了高密度脂蛋白中总胆固醇与血清甘油三酯浓度之间的反曲线关系。当应用于530名男性的单独样本时，极低密度脂蛋白中甘油三酯和低密度脂蛋白中总胆固醇的预测值与超速离心值的相关性与电泳估算值一样好。然而，对于高密度脂蛋白中的总胆固醇，电泳方法更具优势。从同一工业人群的158名女性的超速离心脂蛋白分析中也建立了类似的回归方程。尽管通过电泳和回归方程估算的低密度脂蛋白中总胆固醇浓度在大多数情况下与超速离心值非常接近，但误差超过10%的频率足以限制这些估算在此目的上的价值。在男性和女性中，高密度脂蛋白中总胆固醇与甘油三酯的比值是血清甘油三酯浓度的双曲线函数，这表明随着极低密度脂蛋白中甘油三酯浓度的增加，该脂蛋白核心中的胆固醇酯逐渐被甘油三酯取代。这种非极性脂质组成的改变至少部分解释了高脂血症个体中高密度脂蛋白胆固醇的降低。
