Owens Janel E, Holstege Dirk M, Clifford Andrew J
Department of Nutrition, University of California, Davis, California 95616, USA.
J Agric Food Chem. 2007 May 2;55(9):3737-40. doi: 10.1021/jf063649h. Epub 2007 Mar 31.
An optimal folate nutritional status is important in minimizing developmental and degenerative disease. Therefore, constant monitoring of folate intake and of biomarkers of folate nutritional status is essential. The objective of this research was to compare two folate intake instruments and validate each one against RBC folate measured by a high-throughput liquid chromatography tandem mass spectrometry (HT LC-MS/MS) method described in the companion paper (Owens, J. E.; Holstege, D. M.; Clifford, A. J. J. Agric. Food Chem. 2007, 55, 3292-3297). A food frequency questionnaire (FFQ) and a folate-targeted semiquantitative Block dietary folate equivalents (DFE) screener were compared and individually validated against an HT LC-MS/MS method. RBC folate was 1178 +/- 259 nmol/L (mean +/- SD) in a population of 337 normal adult subjects. Folate intakes were 556 +/- 265 microg/day by the FFQ and 524 +/- 276 microg/day by the DFE screener. Folate intakes by the DFE screener were approximately 34 microg less than by the FFQ (paired t test, p<0.01), but the intake instruments were highly correlated for total folate intake (r=0.608, p<0.01). Correlations between instruments and RBC folate were low (r<0.35) but strong (p<0.01). ROC curve analysis indicates that the measurement of RBC folate by the HT LC-MS/MS method is a better predictive tool than are intake instruments for the evaluation of marginal folate status.
最佳叶酸营养状况对于将发育性疾病和退行性疾病的发生风险降至最低至关重要。因此，持续监测叶酸摄入量和叶酸营养状况生物标志物至关重要。本研究的目的是比较两种叶酸摄入量测量工具，并根据配套论文（Owens, J. E.; Holstege, D. M.; Clifford, A. J. J. Agric. Food Chem. 2007, 55, 3292 - 3297）中描述的高通量液相色谱串联质谱法（HT LC-MS/MS）测定的红细胞叶酸对每种工具进行验证。比较了食物频率问卷（FFQ）和叶酸靶向半定量Block膳食叶酸当量（DFE）筛选器，并分别根据HT LC-MS/MS方法进行验证。在337名正常成年受试者群体中，红细胞叶酸水平为1178±259 nmol/L（平均值±标准差）。通过FFQ测得的叶酸摄入量为556±265 μg/天，通过DFE筛选器测得的叶酸摄入量为524±276 μg/天。DFE筛选器测得的叶酸摄入量比FFQ少约34 μg（配对t检验，p<0.01），但两种摄入量测量工具在总叶酸摄入量方面高度相关（r = 0.608，p<0.01）。工具与红细胞叶酸之间的相关性较低（r<0.35）但很强（p<0.01）。ROC曲线分析表明，与摄入量测量工具相比，HT LC-MS/MS方法测定红细胞叶酸是评估边缘性叶酸状况的更好预测工具。
