Sorouraddin Mohammad H, Amjadi Mohammad, Safi-Shalamzari Mohammad
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Anal Chim Acta. 2007 Apr 18;589(1):84-8. doi: 10.1016/j.aca.2007.02.038. Epub 2007 Feb 22.
This paper describes simple and rapid methods for the fabrication of glass and polymeric chips for routine analytical applications. The methods are easily interfaced to the general laboratory environment and do not require special clean room facilities or expensive instruments. Glass microchips were fabricated by etching with HF solution. Microfluidic channels were designed with CAD program and transferred onto a sheet of commercial polymeric self-adhesive (PSA) film by a cutter plotter. The PSA film was used as a mask for etching process. The etching rate was about 7 microm min(-1). A cover glass plate was sealed on the top of etched substrate by using polycellulose (cellophane). Polymeric microchips were fabricated by sawing with a jigsaw. Commercial polycarbonate (PC) was used as a substrate and two iron sheets were used as leader masks. While this restricts us to the fabrication of straight channels, it is however, much faster and less complicated than the other methods. The chip comprised three polymeric plates and the channels were created in the middle plate. Thermal bonding was used to bond three layers of the microfluidic chip. With this method, we could achieve simple channels with the width of about 200 microm. The channel depth depends on the polymeric plate thickness. Fabricated channels were accurate without any sinuosity or sideshow.
本文描述了用于常规分析应用的玻璃和聚合物芯片的简单快速制造方法。这些方法易于与普通实验室环境对接，不需要特殊的洁净室设施或昂贵的仪器。玻璃微芯片通过用氢氟酸溶液蚀刻来制造。微流控通道用计算机辅助设计（CAD）程序设计，并通过切割机绘图仪转移到一片商用聚合物自粘（PSA）膜上。PSA膜用作蚀刻过程的掩膜。蚀刻速率约为7微米/分钟。通过使用聚纤维素（玻璃纸）将盖玻片密封在蚀刻后的基板顶部。聚合物微芯片通过用锯子锯切来制造。商用聚碳酸酯（PC）用作基板，两片铁片用作导向掩膜。虽然这限制了我们只能制造直通道，但它比其他方法更快且更简单。该芯片由三个聚合物板组成，通道在中间板中形成。热键合用于键合微流控芯片的三层。用这种方法，我们可以实现宽度约为200微米的简单通道。通道深度取决于聚合物板的厚度。制造的通道精确，没有任何弯曲或旁支。
