Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Lab Chip. 2010 Oct 7;10(19):2622-7. doi: 10.1039/c004744a. Epub 2010 Aug 5.
We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the paper or film to form patterns and then sandwich the paper and film between glass sheets or polymer membranes. The hot-melt adhesive wax can realize bridge bonding between various materials, for example, paper, polymethylmethacrylate (PMMA) film, glass sheets, or metal plate. The bonding process is reversible and the wax is reusable through a melting and cooling process. With this process, a three-dimensional (3D) microfluidic chip is achievable by vacuating and venting the chip in a hot-water bath. To study the biocompatibility and applicability of the wax-based microfluidic chip, we tested the PCR compatibility with the chip materials first. Then we applied the wax-paper based microfluidic chip to HeLa cell electroporation (EP). Subsequently, a prototype of a 5-layer 3D chip was fabricated by multilayer wax bonding. To check the sealing ability and the durability of the chip, green fluorescence protein (GFP) recombinant Escherichia coli (E. coli) bacteria were cultured, with which the chemotaxis of E. coli was studied in order to determine the influence of antibiotic ciprofloxacin concentration on the E. coli migration.
我们报告了一种简单、低成本且可分离的微流控芯片，该芯片可将易于获取的纸张、载玻片或其他聚合物薄膜作为芯片材料，并使用热熔胶作为可回收的键合材料。我们使用激光切割纸张或薄膜以形成图案，然后将纸张和薄膜夹在玻璃片或聚合物膜之间。热熔胶蜡可以实现各种材料之间的桥接键合，例如纸张、聚甲基丙烯酸甲酯 (PMMA) 薄膜、玻璃片或金属板。键合过程是可逆的，并且蜡可以通过熔融和冷却过程重复使用。通过该工艺，通过在热水浴中抽空和通风，可实现三维 (3D) 微流控芯片。为了研究基于蜡的微流控芯片的生物相容性和适用性，我们首先测试了芯片材料与 PCR 的兼容性。然后，我们将基于蜡纸的微流控芯片应用于 HeLa 细胞电穿孔 (EP)。随后，通过多层蜡键合制造了 5 层 3D 芯片的原型。为了检查芯片的密封能力和耐用性，培养了绿色荧光蛋白 (GFP) 重组大肠杆菌 (E. coli) 细菌，并用其研究了大肠杆菌的趋化性，以确定抗生素环丙沙星浓度对大肠杆菌迁移的影响。
