Rizzo R, Fuzzi B, Stignani M, Criscuoli L, Melchiorri L, Dabizzi S, Campioni D, Lanza F, Marzola A, Branconi F, Noci I, Baricordi O R
Department of Experimental and Diagnostic Medicine, Section of Medical Genetics, University of Ferrara, Italy.
J Reprod Immunol. 2007 Jun;74(1-2):133-42. doi: 10.1016/j.jri.2007.02.005. Epub 2007 Mar 30.
Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rate, we have investigated the presence of soluble HLA-G molecules in 50 follicular fluids (FFs). The results have shown soluble HLA-G antigens (sHLA-G) in 19/50 (38%) FFs. Furthermore, we have related the presence of sHLA-G molecules in FFs to detection of the soluble antigens in culture supernatants of the corresponding fertilized oocyte, evidencing a significant relationship (p=1.3 x 10(-6); Fisher exact p-test). Specific ELISA and Western blot approaches identified both HLA-G5 and soluble HLA-G1 molecules in FFs while immunocytochemical analysis indicated polymorphonuclear-like and granulosa cells as responsible for production of sHLA-G1 and HLA-G5 molecules. In contrast, only sHLA-G1 antigens were detected in culture supernatants of fertilized oocytes. Overall, these results suggest a role for sHLA-G molecules in the ovulatory process and propose the FFs analysis for sHLA-G molecule presence as a useful tool for oocyte selection in IVF.
目前，体外受精（IVF）程序中采用了不同的方法来选择卵母细胞，但这些方法并不能确保与妊娠结局有显著关联。由于多项研究提出早期胚胎中HLA - G抗原的表达可能是着床率升高的一个标志物，我们研究了50份卵泡液（FFs）中可溶性HLA - G分子的存在情况。结果显示，19/50（38%）的卵泡液中存在可溶性HLA - G抗原（sHLA - G）。此外，我们将卵泡液中sHLA - G分子的存在与相应受精卵母细胞培养上清液中可溶性抗原的检测相关联，证实了两者之间存在显著关系（p = 1.3 x 10(-6)；Fisher精确p检验）。特异性ELISA和蛋白质印迹法在卵泡液中鉴定出了HLA - G5和可溶性HLA - G1分子，而免疫细胞化学分析表明多形核样细胞和颗粒细胞是sHLA - G1和HLA - G5分子的产生来源。相比之下，受精卵母细胞的培养上清液中仅检测到sHLA - G1抗原。总体而言，这些结果表明sHLA - G分子在排卵过程中发挥作用，并提出对卵泡液中sHLA - G分子的存在情况进行分析，作为体外受精中卵母细胞选择的一种有用工具。
