Coenye Tom, Peeters Elke, Nelis Hans J
Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Harelbekestraat 72, B-9000 Gent, Belgium.
Res Microbiol. 2007 May;158(4):386-92. doi: 10.1016/j.resmic.2007.02.001. Epub 2007 Feb 21.
Propionibacterium acnes plays an important role in the pathogenesis of acne vulgaris, a common disorder of the pilosebaceous follicles. Recently, it was suggested that P. acnes cells residing within the follicles grow as a biofilm. In the present study, we tested the biofilm-forming ability of several P. acnes strains in a microtiter plate model. We also evaluated the resistance of biofilm-grown P. acnes towards antimicrobial agents commonly used in the treatment of acne and the production of putative virulence factors. Our results indicate that P. acnes can form biofilms in vitro. The results also show that sessile P. acnes cells are more resistant to various commonly used antimicrobial agents than planktonic cells. In addition, sessile cells produce more extracellular lipases as well as significant amounts of the quorum-sensing molecule autoinducer-2.
痤疮丙酸杆菌在寻常痤疮（一种常见的毛囊皮脂腺疾病）的发病机制中起重要作用。最近，有人提出存在于毛囊内的痤疮丙酸杆菌细胞以生物膜的形式生长。在本研究中，我们在微量滴定板模型中测试了几种痤疮丙酸杆菌菌株的生物膜形成能力。我们还评估了生物膜生长的痤疮丙酸杆菌对痤疮治疗中常用抗菌剂的耐药性以及假定毒力因子的产生。我们的结果表明，痤疮丙酸杆菌可在体外形成生物膜。结果还表明，固着的痤疮丙酸杆菌细胞比浮游细胞对各种常用抗菌剂更具耐药性。此外，固着细胞产生更多的细胞外脂肪酶以及大量的群体感应分子自诱导物-2。
