Pravenec M, Simonet L, Kren V, St Lezin E, Levan G, Szpirer J, Szpirer C, Kurtz T
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Genomics. 1992 Feb;12(2):350-6. doi: 10.1016/0888-7543(92)90384-5.
The rat provides a number of important models of human genetic disease; however, the rat genetic map has not been extensively developed. Although most rat chromosomes carry several gene assignments, some major linkage groups (LG) remain to be mapped. To determine the chromosome location of the largest unmapped linkage group in the rat (LG V containing multiple carboxylesterase loci), we used single-strand conformation polymorphism analysis to identify the rat esterase-10 gene in a panel of rat x mouse somatic cell hybrids. We found that the carboxylesterase gene family and hence LG V are located on rat chromosome 19. We have also confirmed the assignment of the angiotensinogen gene to rat chromosome 19 and have used a large set of recombinant inbred strains to map two anonymous variable number of tandem repeat (VNTR) markers to this chromosome. The current findings bring the total number of genes assigned to rat chromosome 19 from 3 to 19 and provide further evidence of substantial homology between this chromosome and chromosome 8 in the mouse.
大鼠提供了许多重要的人类遗传疾病模型；然而，大鼠遗传图谱尚未得到广泛开发。尽管大多数大鼠染色体上都有几个基因定位，但一些主要的连锁群（LG）仍有待绘制图谱。为了确定大鼠中最大的未绘制图谱的连锁群（包含多个羧酸酯酶基因座的LG V）的染色体位置，我们使用单链构象多态性分析在一组大鼠×小鼠体细胞杂种中鉴定大鼠酯酶-10基因。我们发现羧酸酯酶基因家族以及LG V位于大鼠19号染色体上。我们还证实了血管紧张素原基因定位于大鼠19号染色体，并使用了大量重组近交系将两个匿名可变串联重复序列（VNTR）标记定位到该染色体上。目前的研究结果使定位于大鼠19号染色体的基因总数从3个增加到19个，并进一步证明了该染色体与小鼠8号染色体之间存在大量同源性。
