Du Y, Remmers E F, Zha H, Goldmuntz E A, Szpirer J, Szpirer C, Wilder R L
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Mamm Genome. 1995 Apr;6(4):295-8. doi: 10.1007/BF00352420.
Nine microsatellite loci were mapped to rat Chromosome (Chr) 7 by genetic linkage and somatic cell hybrid analysis. These loci include the gene encoding a member of the IID sub-family of cytochrome P450 (Cyp2d), a gene with repetitive sequences expressed during myotube formation (D7Arb1e), four anonymous loci, D7Arb81, D7Arb208, D7Arb569, D7Arb609a, and three DNA loci defined by MapPair markers R245, R513, and R1071. The nine loci were all identified by PCR-based microsatellite polymorphism analysis and were characterized in 40 F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rats for segregation analysis. These markers formed a single linkage group spanning 76.8 cM with the following order and distances: D7Arb569-11.4 cM-D7Arb81-9.7 cM-R513-2.6 cM-Cyp2d-0.0 cM-R245-1.3 cM-D7Arb1e-10.4 cM-R1071-15.9 cM-D7Arb609a-15.4 cM-D7Arb208. Physical mapping of Cyp2d by somatic cell hybrid analysis allowed us to assign this linkage group to rat Chr 7. For each marker, two to six alleles were detected in a panel of 16 inbred rat strains (ACI/N, BN/SsN, BUF/N, DA/Bkl, F344/N, LER/N, LEW/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, WKY/N).
通过遗传连锁和体细胞杂交分析，将9个微卫星基因座定位到大鼠第7号染色体（Chr 7）上。这些基因座包括编码细胞色素P450（Cyp2d）IID亚家族成员的基因、一个在肌管形成过程中表达的具有重复序列的基因（D7Arb1e）、4个无名基因座D7Arb81、D7Arb208、D7Arb569、D7Arb609a，以及3个由MapPair标记R245、R513和R1071定义的DNA基因座。这9个基因座均通过基于聚合酶链反应（PCR）的微卫星多态性分析进行鉴定，并在Fischer（F344/N）和Lewis（LEW/N）大鼠的40个F2代杂交后代中进行特征分析以进行分离分析。这些标记形成了一个跨度为76.8厘摩（cM）的单一连锁群，顺序和距离如下：D7Arb569 - 11.4 cM - D7Arb81 - 9.7 cM - R513 - 2.6 cM - Cyp2d - 0.0 cM - R245 - 1.3 cM - D7Arb1e - 10.4 cM - R1071 - 15.9 cM - D7Arb609a - 15.4 cM - D7Arb208。通过体细胞杂交分析对Cyp2d进行物理定位，使我们能够将这个连锁群定位到大鼠Chr 7上。对于每个标记，在一组16个近交系大鼠（ACI/N、BN/SsN、BUF/N、DA/Bkl、F344/N、LER/N、LEW/N、LOU/MN、MNR/N、MR/N、SHR/N、SR/Jr、SS/Jr、WBB1/N、WBB2/N、WKY/N）中检测到2至6个等位基因。
