Delporte C, Backhouse N, Inostroza V, Aguirre M C, Peredo N, Silva X, Negrete R, Miranda H F
Laboratory of Natural Products, Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Casilla 233, Santiago 1, Chile.
J Ethnopharmacol. 2007 May 30;112(1):162-5. doi: 10.1016/j.jep.2007.02.018. Epub 2007 Feb 23.
Leaf extracts of Ugni molinae Turcz. (Myrtaceae) are used in Chilean folk medicine as analgesic and anti-inflammatory. The antinociceptive effect of dichloromethane (DCM), ethyl acetate (EA) and methanol (ME) leaf extracts was assessed by intraperitoneal, oral and topical administration in writhing, tail flick, and tail formalin tests in mice. The extracts showed a dose-dependent antinociceptive activity in all the assays under different administration routes. The ED(50) values for the different tests for the DCM, EA, ME extract and reference drug (ibuprofen) were as follows. Writhing test in acetic acid (i.p. administration): 0.21, 0.37, 1.37 and 0.85mg/kg, respectively; tail flick test (oral administration): 199, 189, 120 and 45.9mg/kg. The EC(50) values for tail flick test were (topical administration): 2.0, 0.35, 1.4 and 8.2% (w/v), respectively; and the topical analgesic effects were (formalin assay) 75.5, 77.5, 31.6 and 76.5%, respectively. Ugni molinae extracts produce antinociception in chemical and thermal pain models through a mechanism partially linked to either lipooxygenase and/or cyclooxygenase via the arachidonic acid cascade and/or opioid receptors. Flavonoid glycosides and triterpenoids have been isolated from the plant and can be associated with the observed effect. Our results corroborate the analgesic effects of Ugni molinae, and justify its traditional use for treating pain.
智利草莓（蔷薇科）的叶提取物在智利民间医学中用作止痛和抗炎药。通过腹腔注射、口服和局部给药，在小鼠扭体、甩尾和尾部福尔马林试验中评估了二氯甲烷（DCM）、乙酸乙酯（EA）和甲醇（ME）叶提取物的抗伤害感受作用。在不同给药途径下的所有试验中，提取物均表现出剂量依赖性抗伤害感受活性。DCM、EA、ME提取物和参比药物（布洛芬）在不同试验中的半数有效剂量（ED50）值如下。乙酸扭体试验（腹腔注射给药）：分别为0.21、0.37、1.37和0.85mg/kg；甩尾试验（口服给药）：199、189、120和45.9mg/kg。甩尾试验的半数有效浓度（EC50）值（局部给药）：分别为2.0、0.35、1.4和8.2%（w/v）；局部镇痛效果（福尔马林试验）：分别为75.5、77.5、31.6和76.5%。智利草莓提取物通过与脂氧合酶和/或环氧化酶经由花生四烯酸级联反应和/或阿片受体部分相关的机制，在化学性和热性疼痛模型中产生抗伤害感受作用。已从该植物中分离出黄酮苷和三萜类化合物，它们可能与观察到的作用有关。我们的结果证实了智利草莓的镇痛作用，并证明了其治疗疼痛的传统用途是合理的。
