使用市售试剂选择性检测血浆中的同型半胱氨酸。
Use of a commercially available reagent for the selective detection of homocysteine in plasma.
作者信息
Escobedo Jorge O, Wang Weihua, Strongin Robert M
机构信息
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
出版信息
Nat Protoc. 2006;1(6):2759-62. doi: 10.1038/nprot.2006.399.
Abstract
A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes approximately 30 min.
摘要
描述了一种检测血浆中同型半胱氨酸（Hcy）的方法。将一种市售显色剂添加到血浆样本中。当存在可检测阈值水平的Hcy时，血浆溶液在加热4分钟后会从黄色变为蓝色。不需要色谱分离和免疫原性材料。该方案大约需要30分钟。
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