培养的完整人类神经节的水痘带状疱疹病毒感染
Productive varicella-zoster virus infection of cultured intact human ganglia.
作者信息
Gowrishankar Kavitha, Slobedman Barry, Cunningham Anthony L, Miranda-Saksena Monica, Boadle Ross A, Abendroth Allison
机构信息
Center for Virus Research, Westmead Millenium Institute, and Department of Infectious Diseases and Immunology, University of Sydney, Blackburn Building, 2006 NSW, Australia.
出版信息
J Virol. 2007 Jun;81(12):6752-6. doi: 10.1128/JVI.02793-06. Epub 2007 Apr 4.
Abstract
Varicella-zoster virus (VZV) is a species-specific herpesvirus which infects sensory ganglia. We have developed a model of infection of human intact explant dorsal root ganglia (DRG). Following exposure of DRG to VZV, viral antigens were detected in neurons and nonneuronal cells. Enveloped virions were visualized by transmission electron microscopy in neurons and nonneuronal cells and within the extracellular space. Moreover, rather than remaining highly cell associated during infection of cultured cells, such as fibroblasts, cell-free VZV was released from infected DRG. This model enables VZV infection of ganglionic cells to be studied in the context of intact DRG.
摘要
水痘带状疱疹病毒（VZV）是一种感染感觉神经节的种特异性疱疹病毒。我们建立了人类完整外植体背根神经节（DRG）感染模型。将DRG暴露于VZV后，在神经元和非神经元细胞中检测到病毒抗原。通过透射电子显微镜在神经元、非神经元细胞以及细胞外空间中观察到有包膜的病毒粒子。此外，在感染培养细胞（如成纤维细胞）时，VZV并非高度细胞相关，而是从感染的DRG中释放出游离的VZV。该模型能够在完整DRG的背景下研究VZV对神经节细胞的感染。
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