Yelin D, Boudoux C, Bouma B E, Tearney G J
Wellman Center for Photomedicine, Massachusetts General Hospital, MA 02114, USA.
Opt Lett. 2007 May 1;32(9):1102-4. doi: 10.1364/ol.32.001102.
Imaging large tissue areas with microscopic resolution in vivo may offer an alternative to random excisional biopsy. We present an approach for performing confocal imaging of large tissue surface areas using spectrally encoded confocal microscopy (SECM). We demonstrate a single-optical-fiber SECM apparatus, designed for imaging luminal organs, that is capable of imaging with a transverse resolution of 2.1 microm over a subsurface area of 16 cm2 in less than 1 min. Due to the unique probe configuration and scanning geometry, the speed and resolution of this new imaging technology are sufficient for comprehensively imaging large tissues areas at a microscopic scale in times that are appropriate for clinical use.
在体内以微观分辨率对大组织区域进行成像可能为随机切除活检提供一种替代方法。我们提出了一种使用光谱编码共聚焦显微镜（SECM）对大组织表面积进行共聚焦成像的方法。我们展示了一种专为管腔器官成像设计的单光纤SECM装置，该装置能够在不到1分钟的时间内对16平方厘米的亚表面区域进行横向分辨率为2.1微米的成像。由于独特的探头配置和扫描几何结构，这种新成像技术的速度和分辨率足以在适合临床使用的时间内以微观尺度对大组织区域进行全面成像。
