Khoobehi B, Peyman G A, Vo K
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234.
Ophthalmology. 1992 Jan;99(1):72-9. doi: 10.1016/s0161-6420(92)32007-x.
Liposome-encapsulated fluorescent dye has been altered to allow full fundus angiography after a single laser shot. By exposing the retinal arteries to laser energy where they emerge at the optic disc nerve head, dye release is elicited simultaneously in all four quadrants of the retina, without choroidal fluorescence, allowing visualization of the retinal microcirculation. Because the quantity of dye release is limited, recirculation does not increase background fluorescence. The procedure can be repeated many times without delay with consistent results. Increasing the laser spot size to 1.5 mm allows consistent dye release at energy densities from 0.5 J/cm2 to 3.4 J/cm2. This refinement will allow direct comparison in primates of this technique and conventional fluorescein angiography. The laser energy delivered to the retina is below suggested levels that may be tolerated by the optic nerve.
脂质体包裹的荧光染料已被改进，只需单次激光照射即可完成全眼底血管造影。通过在视盘神经头处将视网膜动脉暴露于激光能量下，可在视网膜的所有四个象限同时引发染料释放，且无脉络膜荧光，从而实现视网膜微循环的可视化。由于染料释放量有限，再循环不会增加背景荧光。该操作可多次重复进行，且不会延迟，结果一致。将激光光斑尺寸增加到1.5毫米可在0.5 J/cm²至3.4 J/cm²的能量密度下实现一致的染料释放。这一改进将使得在灵长类动物中对该技术与传统荧光素血管造影进行直接比较成为可能。传递到视网膜的激光能量低于视神经可能耐受的建议水平。
