在兔中使用扫描激光检眼镜通过荧光素白细胞血管造影术测量视网膜血流。

Measurement of retinal blood flow with fluorescein leucocyte angiography using a scanning laser ophthalmoscope in rabbits.

作者信息

Yang Y, Moon S, Lee S, Kim J

机构信息

Department of Ophthalmology School of Medicine, Wonkwang University, Iksan City, Chonbuk, Korea.

出版信息

Br J Ophthalmol. 1996 May;80(5):475-9. doi: 10.1136/bjo.80.5.475.

DOI:10.1136/bjo.80.5.475

PMID:8695572

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC505502/

Abstract

AIMS

To measure blood flow in the rabbit retinal circulation with fluorescein leucocyte angiography using a scanning laser ophthalmoscope.

METHODS

Blood was withdrawn from the ear vein of a rabbit (New Zealand White), mixed with fluorescent dye in a test tube and centrifuged. The yellow-brown layer containing fluorescein stained leucocytes was collected and injected into the ear vein of the same rabbit while performing fluorescein angiography with a scanning laser ophthalmoscope. The image of retinal angiography displaying circulating fluorescent leucocytes was recorded on video tape. From each frame of the video tape, the consecutive positions of fluorescein stained leucocytes were digitised using an image analysis system and the velocity of blood flow was calculated.

RESULTS

Fluorescent leucocytes were clearly visualised in the retinal arteries, capillaries, and veins which allowed measurement of blood flow. The mean capillary velocity was 0.69 (SD 0.21) mm/s. The mean velocities of leucocytes measured in different sized vessels were as follows: 5.83 (2.42) mm/s in arteries over 50 microns, 3.33 (0.62) mm/s in those 35-50 microns, and 2.42 (1.08) mm/s in arteries under 35 microns, 3.08 (1.56) mm/s in veins over 50 microns, 2.79 (1.49) mm/s in those 35-50 microns, and 1.21 (0.50) mm/s in veins under 35 microns. Blood flow pulsation occurs in arteries, arterioles, veins, and venules but not capillaries.

CONCLUSION

Fluorescein leucocyte angiography can be used for simultaneous measurement of the blood flow in retinal arteries, veins, and capillaries.

摘要

目的

使用扫描激光检眼镜通过荧光素白细胞血管造影术测量兔视网膜循环中的血流。

方法

从一只兔（新西兰白兔）的耳静脉取血，在试管中与荧光染料混合并离心。收集含有荧光素染色白细胞的黄棕色层，并在使用扫描激光检眼镜进行荧光素血管造影时将其注入同一只兔的耳静脉。将显示循环荧光白细胞的视网膜血管造影图像记录在录像带上。使用图像分析系统将录像带每一帧中荧光素染色白细胞的连续位置数字化，并计算血流速度。

结果

在视网膜动脉、毛细血管和静脉中可清晰看到荧光白细胞，从而能够测量血流。平均毛细血管速度为0.69（标准差0.21）mm/s。在不同大小血管中测量的白细胞平均速度如下：直径超过50微米的动脉中为5.83（2.42）mm/s，35 - 50微米的动脉中为3.33（0.62）mm/s，直径小于35微米的动脉中为2.42（1.08）mm/s，直径超过50微米的静脉中为3.08（1.56）mm/s，35 - 50微米的静脉中为2.79（1.49）mm/s，直径小于35微米的静脉中为1.21（0.50）mm/s。动脉、小动脉、静脉和小静脉中会出现血流搏动，但毛细血管中不会。