Tsubota K, Yamada M, Naoi S
Tokyo Dental College, Department of Ophthalmology, Chiba, Japan.
Ophthalmology. 1992 Jan;99(1):89-94. doi: 10.1016/s0161-6420(92)32005-6.
The authors analyzed the specular microscopic appearance of normal corneal epithelium, with particular regard to four cell patterns previously identified in pathologic corneas. Corneal epithelia of 75 normal eyes were examined by specular microscopy using a special compound contact lens. There were no elongated or extra large cells, whereas 44.0% and 35.7% of the corneas showed central epithelial nuclei and irregular patterns, respectively. The mean epithelial cell area and corresponding mean coefficient of variation (CV) for each of the corneas were 595.6 +/- 98.8 microns 2 and 30.3 +/- 15.3%, respectively. The values for the endothelium were 325.7 +/- 48.4 microns 2 and 33.6 +/- 6.3%, respectively. Although there was a significant Pearson correlation (r = 0.50, P less than 0.01) between mean endothelial cell area and age, none existed (r = 0.19, P greater than 0.05) between mean epithelial cell area and age, even though there was a relationship (r = 0.35, P less than 0.01) between the mean cell areas themselves. There were no significant relationships among the CV of the epithelium, that of the endothelium, and age. This study presents a powerful new technique and baseline data by which to assess normal corneal epithelium and any aberration.
作者分析了正常角膜上皮的镜面显微镜外观，特别关注先前在病理性角膜中确定的四种细胞模式。使用特殊的复合接触镜通过镜面显微镜检查了75只正常眼睛的角膜上皮。没有发现拉长或超大细胞，而分别有44.0%和35.7%的角膜显示中央上皮细胞核和不规则模式。每个角膜的平均上皮细胞面积和相应的平均变异系数（CV）分别为595.6±98.8平方微米和30.3±15.3%。内皮细胞的值分别为325.7±48.4平方微米和33.6±6.3%。虽然平均内皮细胞面积与年龄之间存在显著的Pearson相关性（r = 0.50，P < 0.01），但平均上皮细胞面积与年龄之间不存在相关性（r = 0.19，P > 0.05），尽管平均细胞面积本身之间存在关系（r = 0.35，P < 0.01）。上皮细胞的CV、内皮细胞的CV和年龄之间没有显著关系。本研究提出了一种强大的新技术和基线数据，可用于评估正常角膜上皮和任何异常情况。
